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| Status |
Public on May 05, 2022 |
| Title |
Transcriptome analyses of Pst DC3000-inoculated Arabidopsis thaliana Col-0 and 35S::CBP60g plants at normal and elevated temperature |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed RNA sequencing of Pseudomonas syringae pv. tomato (Pst) DC3000-infected A. thaliana Col-0 and 35S::CBP60g plants at normal (23C) and elevated (28C) temperatures. 4-week-old plants were pre-incubated at 23C and 28C for 48h and then leaves were syringe-infiltrated with DC3000 bacterial inoculum. Plants were incubated at their respective temperatures (23C or 28C) for another 24h post-inoculation before tissue collection for RNA extraction. RNA samples for submitted for RNA sequencing and we found different clusters of DC3000-regulated genes that were similarly or differentially regulated between Col-0 and 35S::CBP60g at elevated temperature. Temperature-downregulated DC3000-induced genes in Col-0 plants that were restored in 35S::CBP60g plants were enriched for immunity/defense-related genes, including those essential for host salicylic acid (defense hormone) biosynthesis and accumulation.
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| Overall design |
4 treatments were used: (1) 23C DC3000 Col-0; (2) 23C DC3000 35S::CBP60g; (3) 28C DC3000 Col-0; and (4) 28C DC3000 35S::CBP60g. For each treatment, three individual plants (independent biological replicates) were sampled.
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| Contributor(s) |
Kim JH, Castroverde CM, He SY |
| Citation(s) |
35768511 |
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| Submission date |
Mar 02, 2022 |
| Last update date |
Jul 02, 2022 |
| Contact name |
Jonghum Kim |
| E-mail(s) |
qmanis@yonsei.ac.kr
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| Phone |
5177037290
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| Organization name |
Duke university
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| Department |
Biology
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| Lab |
Sheng Yang He lab
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| Street address |
124 Science Dr
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| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27701 |
| Country |
USA |
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| Platforms (1) |
| GPL21785 |
Illumina HiSeq 4000 (Arabidopsis thaliana) |
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| Samples (12)
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| GSM5929894 |
Col-0, 28C, DC3000 Rep 1 |
| GSM5929895 |
Col-0, 28C, DC3000 Rep 2 |
| GSM5929896 |
Col-0, 28C, DC3000 Rep 3 |
| GSM5929897 |
35S::CBP60g, 23C, DC3000 Rep 1 |
| GSM5929898 |
35S::CBP60g, 23C, DC3000 Rep 2 |
| GSM5929899 |
35S::CBP60g, 23C, DC3000 Rep 3 |
| GSM5929900 |
35S::CBP60g, 28C, DC3000 Rep 1 |
| GSM5929901 |
35S::CBP60g, 28C, DC3000 Rep 2 |
| GSM5929902 |
35S::CBP60g, 28C, DC3000 Rep 3 |
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| Relations |
| BioProject |
PRJNA812018 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE197771_ProcessedData_Castroverde_Kim_2022_CBP60g_RNA_Seq.csv.gz |
1.2 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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