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Status |
Public on May 29, 2023 |
Title |
Low temperature and mTOR inhibition favor stem cell maintenance in human keratinocyte cultures [miRNA-seq] |
Organism |
Homo sapiens |
Experiment type |
Non-coding RNA profiling by high throughput sequencing
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Summary |
Purpose: Comparison of genes expression pattern in normal human epidermal keratinocytes (NHEKs) which were cultured at several specific temperatures or with rapamycin. Methods: Basically, NHEKs were cultured with irradiated 3T3J2 feeder cells in cFAD medium (Pr Green's method) for 1 week. NHEKs were cultured at 32, 35, 36, 37 and 38 degrees C from beginning to end of cell culture, respectively. Furthermore, 100 nM rapamycin-added cFAD medium and ed at 37 degrees C. 7days after cell cuture, NHEKs were harvested for RNAseq analysis. Results: The culture condition at 32 degrees C and 37 degrees C with rapamycin maintained human epidermal keratinocyte stem cells. Conclusions: mTORC1 inhibition via temperature changes favors ex vivo maintenance of human epidermal keratinocyte stem cells.
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Overall design |
miRNA profiles in human epidermal keratinocytes which were cultured at some specific temperatures or with 100 nM rapamycin.
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Contributor(s) |
Barrandon Y, Sakabe J, Nanba D, Shimokawa M |
Citation(s) |
37139607 |
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Submission date |
Mar 15, 2022 |
Last update date |
Sep 07, 2023 |
Contact name |
Jun-Ichi Sakabe |
E-mail(s) |
sakabe.junichi@sgh.com.sg
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Phone |
91003620
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Organization name |
Singapore General Hospital (SGH)
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Department |
Plastic Reconstructive and Aesthetic Surgery
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Lab |
Skin Bank Unit
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Street address |
Blk4 Lv3, Outram Rd
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City |
Singapore |
ZIP/Postal code |
169608 |
Country |
Singapore |
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Platforms (1) |
GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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Samples (6)
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GSM5955179 |
Cultured normal human epidermal keratinocytes at 32 degrees C (miRNA) |
GSM5955180 |
Cultured normal human epidermal keratinocytes at 35 degrees C (miRNA) |
GSM5955181 |
Cultured normal human epidermal keratinocytes at 36 degrees C (miRNA) |
GSM5955182 |
Cultured normal human epidermal keratinocytes at 37 degrees C (miRNA) |
GSM5955183 |
Cultured normal human epidermal keratinocytes at 38 degrees C (miRNA) |
GSM5955184 |
Cultured normal human epidermal keratinocytes with 100 nM Rapamycin at 37 degrees C (miRNA) |
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This SubSeries is part of SuperSeries: |
GSE199523 |
Low temperature and mTOR inhibition favor stem cell maintenance in human keratinocyte cultures |
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Relations |
BioProject |
PRJNA816483 |