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| Status |
Public on May 29, 2023 |
| Title |
Low temperature and mTOR inhibition favor stem cell maintenance in human keratinocyte cultures [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: Comparison of genes expression pattern in normal human epidermal keratinocytes (NHEKs) which were cultured at several specific temperatures or with rapamycin.
Methods: Basically, NHEKs were cultured with irradiated 3T3J2 feeder cells in cFAD medium (Pr Green's method) for 1 week. NHEKs were cultured at 32, 35, 36, 37 and 38 degrees C from beginning to end of cell culture, respectively. Furthermore, 100 nM rapamycin-added cFAD medium and ed at 37 degrees C. 7days after cell cuture, NHEKs were harvested for RNAseq analysis.
Results: The culture condition at 32 degrees C and 37 degrees C with rapamycin maintained human epidermal keratinocyte stem cells.
Conclusions: mTORC1 inhibition via temperature changes favors ex vivo maintenance of human epidermal keratinocyte stem cells.
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| |
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| Overall design |
mRNA profiles in human epidermal keratinocytes which were cultured at some specific temperatures or with 100 nM rapamycin.
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| |
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| Contributor(s) |
Barrandon Y, Sakabe J, Nanba D, Shimokawa M |
| Citation(s) |
37139607 |
| |
| Submission date |
Mar 28, 2022 |
| Last update date |
Sep 07, 2023 |
| Contact name |
Jun-Ichi Sakabe |
| E-mail(s) |
sakabe.junichi@sgh.com.sg
|
| Phone |
91003620
|
| Organization name |
Singapore General Hospital (SGH)
|
| Department |
Plastic Reconstructive and Aesthetic Surgery
|
| Lab |
Skin Bank Unit
|
| Street address |
Blk4 Lv3, Outram Rd
|
| City |
Singapore |
| ZIP/Postal code |
169608 |
| Country |
Singapore |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (6)
|
| GSM5974606 |
Cultured normal human epidermal keratinocytes at 32 degrees C (mRNA) |
| GSM5974607 |
Cultured normal human epidermal keratinocytes at 35 degrees C (mRNA) |
| GSM5974608 |
Cultured normal human epidermal keratinocytes at 36 degrees C (mRNA) |
| GSM5974609 |
Cultured normal human epidermal keratinocytes at 37 degrees C (mRNA) |
| GSM5974610 |
Cultured normal human epidermal keratinocytes at 38 degrees C (mRNA) |
| GSM5974611 |
Cultured normal human epidermal keratinocytes with 100 nM Rapamycin at 37 degrees C (mRNA) |
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| This SubSeries is part of SuperSeries: |
| GSE199523 |
Low temperature and mTOR inhibition favor stem cell maintenance in human keratinocyte cultures |
|
| Relations |
| BioProject |
PRJNA820608 |
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