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| Status |
Public on Aug 01, 2022 |
| Title |
Transcriptome of a Synthetic Microbial Community |
| Organisms |
Vibrio hepatarius; Ruegeria pomeroyi DSS-3; Thalassospira sp. HF15 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To explore the ecological basis for multiple bacteria species coexistence, we set up three bacteria (Ruegeria pomeroyi DSS-3, Vibrio hepatarius HF70, and Thalassospira sp. HF15), either in monoculture or in co-cultures (in all combinations) for a 8 day growth-dilution cycles. At ~15h of day 4 (P4) and day 8 (P8) of growth-dilution cycles, we examined transcriptomic responses of these bacteria. Differential gene expressions were used to generate hypothesis about ecological and physiological responses of one in the presence of another/other bacteria.
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| Overall design |
mRNA profile of three bacteria under different conditions
Please note that mono-culture samples of three bacterial species were pooled together for RNA-seq library prep and sequencing run.
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| Contributor(s) |
Fu H, Moran MA, Smith CB |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 30, 2022 |
| Last update date |
Oct 28, 2022 |
| Contact name |
He Fu |
| E-mail(s) |
biofuhe@gmail.com
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| Organization name |
University of Georgia
|
| Department |
Microbiology
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| Street address |
120 Cedar St
|
| City |
Athens |
| State/province |
GA |
| ZIP/Postal code |
30602 |
| Country |
USA |
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| Platforms (4)
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| GPL32094 |
Illumina NextSeq 500 (Ruegeria pomeroyi DSS-3; Thalassospira sp. HF15; Vibrio hepatarius) |
| GPL32095 |
Illumina NextSeq 500 (Ruegeria pomeroyi DSS-3; Vibrio hepatarius) |
| GPL32096 |
Illumina NextSeq 500 (Thalassospira sp. HF15; Vibrio hepatarius) |
| GPL32097 |
Illumina NextSeq 500 (Ruegeria pomeroyi DSS-3; Thalassospira sp. HF15) |
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| Samples (40)
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| GSM5984927 |
pooled monoculture (Rpom_Vib70_Tha15) P4 rep1 |
| GSM5984928 |
pooled monoculture (Rpom_Vib70_Tha15) P4 rep2 |
| GSM5984929 |
pooled monoculture (Rpom_Vib70_Tha15) P4 rep3 |
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| Relations |
| BioProject |
PRJNA821508 |
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