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| Status |
Public on Jun 30, 2010 |
| Title |
Gene expression profie of OSMF (Oral Submucous Fibrosis) tissues compared with the expression in normal oral tissues |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
ABSTRACT:
Background: Oral Submucous Fibrosis (OSF) is a chronic inflammatory disease resulting in progressive fibrosis of the oral soft tissues. Habit of chewing betel quid has been proposed as an important etiological factor in the development of this disease. But the exact mechanism of pathogenesis is still not clear.
Methods: We took microarray approach to identify differentially regulated genes in 10 OSF tissues against 8 pooled normal tissues using oligonucleotide arrays. Microarray results have been confirmed by qRT-PCR. Regulation of genes in epithelial and fibroblast cells was studied after treatment with arecoline, TGF-b and LAP followed by qRT-PCR.
Results: Total number of genes found to be commonly regulated in OSF (p<=0.05 and Fold change>=1.5) tissues are 5288 and among them 2884 are up-regulated and 2404 are down-regulated. Extra cellular matrix genes that have been reported in OSF such as collagens, fibronectin and cytokines ET1, CTGF and TGF-b1 are among the up regulated genes. The list of up and down regulated genes also includes RARRES1, TGM2, THBS1, CHGB, MMP3, SPP1 and ALOX12, C4orf7 respectively. Analysis of microarray data revealed TGF-? pathway as a principal gene network involved in the development of OSF. Intriguingly, there was no regulation of CTGF and THBS1 in fibroblasts by arecoline. However, TGF-b1 treatment regulated all these genes in both epithelial and fibroblast cells.
Conclusion: We demonstrate over expression of novel genes in OSF and suggest that OSF development involves TGF-b pathway in an epithelial-stromal cooperation. Targeting TGF-b signaling could be an alternate strategy to treat OSF.
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| Overall design |
Two-condition experiment: 10 OSF tissues against 8 pooled normal tissues
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| Contributor(s) |
Khan I, Agarwal P, Kondaiah P |
| Citation(s) |
21591998 |
| |
| Submission date |
Feb 03, 2010 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Paturu Kondaiah |
| E-mail(s) |
paturu@mrdg.iisc.ernet.in
|
| Phone |
080-22932688
|
| Organization name |
IISc
|
| Department |
MRDG
|
| Lab |
Cancer Biology
|
| Street address |
CV Raman Avenue
|
| City |
Bangalore |
| State/province |
Karnataka |
| ZIP/Postal code |
560012 |
| Country |
India |
| |
|
| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
|
| Samples (10)
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| GSM506108 |
pooled normals vs 63 number sample |
| GSM506109 |
pooled normals vs 65 number sample |
| GSM506110 |
pooled normals vs 66 number sample |
| GSM506111 |
pooled normals vs 67 number sample |
| GSM506112 |
pooled normals vs 78 number sample |
| GSM506113 |
pooled normals vs 125 number sample |
| GSM506114 |
pooled normals vs 130 number sample |
|
| Relations |
| BioProject |
PRJNA125633 |
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