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| Status |
Public on Dec 16, 2022 |
| Title |
A role for gene expression and mRNA stability in nutritional compensation of the circadian clock |
| Organism |
Neurospora crassa |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To identify the role of the cleavage and polyadenylation CFIm complex (NCU02152 and NCU09014) in the circadian clock, mRNA 3' End Sequencing was performed on a knockout mutant and compared to control samples.
Hundreds of polyadenylation sites are affected by loss of CFIm complex activity in Neurospora crassa.
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| Overall design |
3' End Sequencing was performed on biological duplicate samples from 25C, constant light cultures grown for 72 hours to identify poly(A) tail locations.
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| Contributor(s) |
Kelliher CM, Stevenson E, Loros JJ, Dunlap JC |
| Citation(s) |
36603054 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| F32 GM128252 |
Genetic and Molecular Dissection of Regulatory Mechanisms Underlying Temperature and Nutritional Compensation of the Circadian Clock in Neurospora crassa |
DARTMOUTH COLLEGE |
Christina Marie Kelliher |
| R35 GM118021 |
Genetic and Molecular Dissection of the Neurospora Clock |
DARTMOUTH COLLEGE |
Jay C. Dunlap |
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| Submission date |
Apr 29, 2022 |
| Last update date |
Feb 03, 2023 |
| Contact name |
Christina M Kelliher |
| E-mail(s) |
Christina.M.Kelliher@dartmouth.edu
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| Organization name |
Geisel School of Medicine at Dartmouth
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| Department |
Molecular & Systems Biology
|
| Street address |
7400 Hinman
|
| City |
Hanover |
| State/province |
NH |
| ZIP/Postal code |
03755 |
| Country |
USA |
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| Platforms (1) |
| GPL32221 |
Illumina MiniSeq (Neurospora crassa) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA833430 |
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