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| Status |
Public on Dec 31, 2022 |
| Title |
Decellularized Spinal Cord Matrix Manipulate Glial Niche Alteration via Serglycin Mediated Signalling Pathway [scRNA-seq] |
| Organisms |
Homo sapiens; Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The decellularized spinal cord matrix (DSCM) provides the advantage in the translational potential for microenvironment replacement therapies to repair spinal cord injury (SCI) However, the cell type alteration in the SCI repairing niche is undefined. Using single cell transcriptome and bulk RNA-seq analysis, we showed that DSCM regulated cell fate changes in niches related to the multicellular model. This work opens the door for understanding the SCI repairing from a tissue niche perspective.
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| Overall design |
We evaluated and compared the cells migrated out from spinal cord slice, hspASCs, and ECs under DSCM and Collagen I (COLI) culture conditions using organotypic model and human hspASC-hEC co-culture model.
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| Contributor(s) |
Zhang S, Zhang X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 11, 2022 |
| Last update date |
Jan 02, 2023 |
| Contact name |
Sheng Zhang |
| E-mail(s) |
zhang_sheng@gibh.ac.cn
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| Organization name |
School of Materials Science and Engineering, Sun Yat-sen University
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| Street address |
132 Waihuan West Road, Guangzhou Science Park, Luogang District
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| City |
Guangzhou |
| State/province |
None Selected |
| ZIP/Postal code |
510006 |
| Country |
China |
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| Platforms (2) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
| GPL25947 |
Illumina NovaSeq 6000 (Rattus norvegicus) |
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| Samples (5)
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| This SubSeries is part of SuperSeries: |
| GSE202733 |
Decellularized Spinal Cord Matrix Manipulate Glial Niche Alteration via Serglycin Mediated Signalling Pathway |
|
| Relations |
| BioProject |
PRJNA842511 |
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