|
| Status |
Public on Apr 02, 2010 |
| Title |
Promoter proximal pausing and its regulation by c-Myc in embryonic stem cells: ChIP-chip |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
Recruitment of the RNA Polymerase II (Pol II) transcription initiation apparatus to promoters by specific DNA binding transcription factors is well recognized as a key regulatory step in gene expression. We describe here evidence that promoter-proximal pausing is a general feature of transcription by Pol II in embryonic stem (ES) cells, and thus an additional step where regulation of gene expression may occur. We report here that c-Myc, which occupies a third of actively transcribed genes in ES cells and is a key regulator of cellular proliferation, binds P-TEFb and contributes to release of promoter-proximal paused Pol II at these genes.
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| |
|
| Overall design |
ChIP-chip data for Pol II and additional factors controlling pause release in mouse ES cells.
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| |
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| Contributor(s) |
Rahl PB, Lin CY, Seila AC, Flynn RA, McCuine S, Burge C, Sharp PA, Young RA |
| Citation(s) |
20434984 |
| |
| Submission date |
Feb 25, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Charles Lin |
| E-mail(s) |
chazlin@mit.edu
|
| Phone |
6172585218
|
| Fax |
6172580376
|
| URL |
http://web.wi.mit.edu/young/
|
| Organization name |
Massachusetts Institute of Technology
|
| Department |
Biology
|
| Lab |
Richard A. Young
|
| Street address |
9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL10099 |
MIT M. Musculus Promoter array 44K |
|
| Samples (8)
|
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| This SubSeries is part of SuperSeries: |
| GSE20485 |
Promoter proximal pausing and its regulation by c-Myc in embryonic stem cells |
|
| Relations |
| BioProject |
PRJNA129863 |
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