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| Status |
Public on Jun 15, 2010 |
| Title |
Effects of RGMc on BMP2 and BMP6-mediated Gene Expression in Hep3B Liver Cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Mutations in repulsive guidance molecule c (RGMc) / hemojuvelin (HJV) cause juvenile hemochromatosis, an aggravated iron overload disorder that presents early in life. Patients with juvenile hemochromatosis, and RGMc knockout mice, have diminished expression of the key iron-regulatory peptide, hepcidin. This suggests that RGMc plays a critical role in the regulation of iron homeostasis; however the mechanisms of RGMc actions are unknown. Recent studies have shown that RGMc directly binds to the growth factors, bone morphogenetic protein 2 and 6 (BMP2 and BMP6), and it is possible that this interaction regulates aspects of iron metabolism.
We used microarrays to examine the effects of RGMc on BMP2- and BMP6-mediated gene expression.
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| Overall design |
In our experimental model we treated Hep3B liver cells that had been serum starved for 16 hours as follows: (1) un-treated, (2) BMP2, (3) BMP2 + 10-fold molar excess of Noggin (a potent BMP2 inhibitor), (4) BMP2 + 20-fold molar excess of RGMc, (5) BMP6, (6) BMP6 + 10-fold molar excess of Noggin, (7) BMP6 + 20-fold molar excess of RGMc. Binding was allowed to proceed for BMP and Noggin or RGMc for 3 hr at 20°C prior to treatment of Hep3B cells. Treatment time was 4 hr at which cells were collected for RNA isolation.
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| Contributor(s) |
Nili M, Rotwein P |
| Citation(s) |
20530805 |
| |
| Submission date |
Mar 08, 2010 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Mahta Nili |
| Organization name |
Oregon Health & Science University
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| Department |
Biochemistry and Molecular Biology
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| Street address |
3181 SW Sam Jackson Park Road
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| City |
Portland |
| State/province |
OR |
| ZIP/Postal code |
97239 |
| Country |
USA |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA124929 |
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