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| Status |
Public on Mar 16, 2010 |
| Title |
MicroRNA expression profiling of the porcine developing brain |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human betaherpesvirus 5; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Human gammaherpesvirus 8; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Sus scrofa |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
MicroRNA expression profiling with use of microRNA microarrays was performed on porcine developing brain. Two tissues, cortex and cerebellum were sampled at each of three different developmental stages: gestation day 50 (F50), gestation day 100 (F100) and three months old (named adult), respectively. The aim was to find developmental stage as well as the tissue specific microRNA candidates and validate them with qPCR.
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| Overall design |
Samples containing both small RNA and total RNA were subjected, respectively. One replicate per array was made from each developmental stage/tissue, for each isolation method. Fetus gestation day 50 cortex total RNA was run in four replicates. Fetus gestation day 50 cortex small RNA was run in two replicates.
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| Contributor(s) |
Podolska A, Kaczkowski B, Busk PK, Litman T, Cirera S, Fredholm M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 15, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Agnieszka Podolska |
| E-mail(s) |
agap@life.ku.dk
|
| Phone |
+4535333051
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| Organization name |
Faculty of Life Sciences, University of Copenhagen,
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| Department |
Department of Basic Animal and Veterinary Sciences
|
| Street address |
Grønnegårdsvej 3 2nd floor
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| City |
Frederiksberg C |
| ZIP/Postal code |
1870 |
| Country |
Denmark |
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| Platforms (1) |
| GPL7724 |
miRCURY LNA microRNA Array, v. 9.2, all organisms |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA124745 |
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