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| Status |
Public on Feb 03, 2023 |
| Title |
Chemical reprogramming of melanocytes to skeletal muscle cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We identify a combination of small molecules to be able to transdifferentiate dermal cells to muscle cells. To characterize the cellular diversity of the neonatal dermal we performed scRNA-seq on mononuclear cell suspensions obtained from the neonatal dermal. Following normalization and quality control of scRNA-seq data, we captured 40537 cells with an average of 5755 genes expressed per cell. We charted the entire course of skin-to-muscle chemical reprogramming by single cell RNA-sequencing to determine the source cells in CD73- cells from skin with the potential to be transdifferentiated to muscle cells .We found that melanocytes could be chemically reprogrammed to myogenic lineage cells.
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| Overall design |
Single cell RNA profiles from neonatal CD73- dermal cells treated by small molecules
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| Contributor(s) |
Hu P, Yang W, Li C, Wang Y |
| Citation(s) |
36726338 |
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| Submission date |
Sep 12, 2022 |
| Last update date |
Feb 05, 2023 |
| Contact name |
Yaqi Wang |
| E-mail(s) |
yaqi_wang@pku.edu.cn
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| Organization name |
Peking University
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| Street address |
No.5 Yiheyuan Road Haidian District
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA879735 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE213195_RAW.tar |
1.2 Gb |
(http)(custom) |
TAR (of CSV, MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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