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Series GSE213682 Query DataSets for GSE213682
Status Public on Nov 25, 2022
Title Zygotic genome activation by the totipotency pioneer factor Nr5a2 IV
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Life begins with a switch in genetic control from the maternal to the embryonic genome during zygotic genome activation (ZGA). Despite its importance, the essential regulators of ZGA remain largely unknown in mammals. Based on de novo motif searches, we identified the orphan nuclear receptor Nr5a2 as a key activator of major ZGA in mouse 2-cell embryos. Nr5a2 is required for progression beyond the 2-cell stage. It binds to its motif within SINE B1/Alu retrotransposable elements found in cis-regulatory regions of ZGA genes. Chemical inhibition suggests that 72% of ZGA genes are regulated by Nr5a2 and potentially other orphan nuclear receptors. Nr5a2 promotes chromatin accessibility during ZGA and binds nucleosomal DNA in vitro. We conclude that Nr5a2 is an essential pioneer factor that regulates ZGA.
 
Overall design Single embryo RNA seq of SR1848 treated (41), SR1848 + Nr5a2 OE (67) and DMSO control (29) 2C embryos.
 
Contributor(s) Gassler J, Kobayashi W, Gáspár I, Ruangroengkulrith S, Mohanan A, Hernandez LG, Kravchenko P, Kümmecke M, Lalic A, Ashburn RJ, Rifel N, Zaczek M, Vallot A, Rico LC, Ladstätter S, Tachibana K
Citation(s) 36423263
Submission date Sep 19, 2022
Last update date Nov 25, 2022
Contact name Pavel Andreevich Kravchenko
E-mail(s) pkravchenko@biochem.mpg.de
Phone +4915110675513
Organization name Max-Planck-Institute of Biochemistry
Department Totipotency
Lab Tachibana
Street address Am Klopferspitz 18
City Munich
State/province Planegg
ZIP/Postal code 82152
Country Germany
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (137)
GSM6591883 Single 2 cell embryo. Sample 10 ex1 Condition: DMSO
GSM6591884 Single 2 cell embryo. Sample 10 ex2 Condition: DMSO
GSM6591885 Single 2 cell embryo. Sample 11 ex1 Condition: DMSO
This SubSeries is part of SuperSeries:
GSE178661 Zygotic genome activation by the totipotency pioneer factor Nr5a2
Relations
BioProject PRJNA882060

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Supplementary file Size Download File type/resource
GSE213682_DESeq2_FDR0.05_SR1848vsSR1848_Nr5a2OE_transcripts.csv.gz 4.1 Mb (ftp)(http) CSV
GSE213682_txi.kallisto_counts_raw.csv.gz 19.6 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record
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