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| Status |
Public on Oct 01, 2023 |
| Title |
In-Depth Characterization of Cell Subculture Process based on Multi-Omics |
| Organism |
Chlorocebus aethiops |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investegate the mechanism of physiological changes during cell digestion, we analyzed the levels of RNAs in cells treated with animal-based enzymes and animal origin-free enzymes.
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| Overall design |
The Vero cells were divided into 3 groups. The first group (Group 1) was digested with trypsin at 37°C. The cells collected immediately after digestion were set to 0 h, and the digested cells were placed back in the cell incubator for 8 hours. After the cells were digested with non-enzymatic digestion solution and then collected for 8 h, the cells af-ter trypsinization at 37°C were further cultured for 24 hours, and the cells were di-gested with non-enzymatic digestion solution and collected for 24 h. The second group (Group 2) was digested with TrypLE at 37°C, then set the cells collected immediately after digestion as 0 h, place the digested cells back in the cell incubator for 8 hours, and digest the recovered cells with non-enzyme digestion solution. Set it to 8h, continue to culture the cells digested with TrypLE digestion solution at 37°C for 24 hours, and then digest the cells with non-enzyme digestion solution for 24h. The third group (Group 3) used 4°C TrypLE digestion solution at 25°C digestion, the cells collected immediately after digestion were set to 0 h, and the digested cells were placed back in the cell incu-bator for 8 hours, and the cells collected after digestion with non-enzyme digestion solution were set to 8 h, and digested with TrypLE digestion solution at 4°C. After the cells were cultured for 24 hours, the collected cells were digested with non-enzymatic digestion solution and set to 24 hours. In addition, Vero cells were digested with an enzyme-free digestion solution at 37°C, and the immediately harvested cells were set as the control group. Each sample has three biological replicates.
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| Contributor(s) |
Yu S, Huang Y, Wu C, Fu W, Liang H, Chen C, Cheng Y, Guo Y, Zhang Y, Wang H, Yang X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Oct 15, 2022 |
| Last update date |
Oct 01, 2023 |
| Contact name |
Yunchao Huang |
| E-mail(s) |
ychaohuang@bjmu.edu.cn
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| Organization name |
Beijing Institute of Biological Products Company Limited
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| Street address |
Boxing 2nd Road
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| City |
Beijing |
| ZIP/Postal code |
100176 |
| Country |
China |
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| Platforms (1) |
| GPL29682 |
Illumina NovaSeq 6000 (Chlorocebus aethiops) |
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| Samples (30)
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| GSM6643098 |
Vero cells, Group1, 0h, rep1 |
| GSM6643099 |
Vero cells, Group1, 0h, rep2 |
| GSM6643100 |
Vero cells, Group1, 0h, rep3 |
| GSM6643101 |
Vero cells, Group1, 8h, rep1 |
| GSM6643102 |
Vero cells, Group1, 8h, rep2 |
| GSM6643103 |
Vero cells, Group1, 8h, rep3 |
| GSM6643104 |
Vero cells, Group1, 24h, rep1 |
| GSM6643105 |
Vero cells, Group1, 24h, rep2 |
| GSM6643106 |
Vero cells, Group1, 24h, rep3 |
| GSM6643107 |
Vero cells, Group2, 0h, rep1 |
| GSM6643108 |
Vero cells, Group2, 0h, rep2 |
| GSM6643109 |
Vero cells, Group2, 0h, rep3 |
| GSM6643110 |
Vero cells, Group2, 8h, rep1 |
| GSM6643111 |
Vero cells, Group2, 8h, rep2 |
| GSM6643112 |
Vero cells, Group2, 8h, rep3 |
| GSM6643113 |
Vero cells, Group2, 24h, rep1 |
| GSM6643114 |
Vero cells, Group2, 24h, rep2 |
| GSM6643115 |
Vero cells, Group2, 24h, rep3 |
| GSM6643116 |
Vero cells, Group3, 0h, rep1 |
| GSM6643117 |
Vero cells, Group3, 0h, rep2 |
| GSM6643118 |
Vero cells, Group3, 0h, rep3 |
| GSM6643119 |
Vero cells, Group3, 8h, rep1 |
| GSM6643120 |
Vero cells, Group3, 8h, rep2 |
| GSM6643121 |
Vero cells, Group3, 8h, rep3 |
| GSM6643122 |
Vero cells, Group3, 24h, rep1 |
| GSM6643123 |
Vero cells, Group3, 24h, rep2 |
| GSM6643124 |
Vero cells, Group3, 24h, rep3 |
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| Relations |
| BioProject |
PRJNA890891 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE215840_G1_0hvsG2_0h_all_KEGGenrich.xls.gz |
23.2 Kb |
(ftp)(http) |
XLS |
| GSE215840_G1_0hvsG2_0h_all_KEGGenrich_significant.xls.gz |
2.5 Kb |
(ftp)(http) |
XLS |
| GSE215840_G1_0hvsG2_0h_deg_all.xls.gz |
236.5 Kb |
(ftp)(http) |
XLS |
| GSE215840_Union_for_cluster.xls.gz |
23.3 Kb |
(ftp)(http) |
XLS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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