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| Status |
Public on May 12, 2010 |
| Title |
CD62L expression identifies a unique subset of polyfunctional CD56dim NK cells: Groups 1-3 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Human Natural Killer (NK) cells comprise two main subsets, CD56bright and CD56dim cells, that differ in function, phenotype and tissue localization. To further dissect the heterogeneity of CD56dim cells, we have performed transcriptome analysis and functional ex vivo characterization of human NK cell subsets according to the expression of markers related to differentiation, migration or competence. Here, we show for the first time that the ability to respond to cytokines or to activating receptors is mutually exclusive in almost all NK cells with the exception of CD56dim CD62L+ cells. Indeed, only these cells combine the ability to produce interferon (IFN)-gamma after cytokines and proliferate in vivo during viral infection with the capacity to kill and produce cytokines upon engagement of activating receptors. Therefore, CD56dim CD62L+ cells represent a unique subset of polyfunctional NK cells. Ex vivo analysis of their function, phenotype, telomere length, frequencies during ageing as well as transfer experiments of NK cell subsets into immunodeficient mice suggest that CD56dim CD62L+ cells represent an intermediate stage of NK cell maturation, which after restimulation can accomplish multiple tasks and further develop into terminally differentiated effectors.
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| Overall design |
Gene expression profiles of FACSAria sorted CD3- CD56bright CD62L+, CD3- CD56dim CD62L+ and CD3- CD56dim CD62L- NK cells from human peripheral blood of three donors were compared using Affymetrix GeneChip Human Genome HG-U133_Plus_2. After total RNA extraction, reverse transcription, cDNA extraction, the biotinylated cRNA was transcribed, fragmented, and 15 µg cRNA hybridized in triplicates for each of the three groups to the GeneChip arrays. Group1: CD3- CD56bright CD62L+,.Group2: CD3- CD56dim CD62L+, Group3: CD3- CD56dim CD62L-. Lists of differentially regulated genes were created using High Performance Chip Data Analysis (HPCDA) with Bioretis database (http://www.bioretis-analysis.de). Worldwide data sharing is possible via Bioretis, please ask the authors.
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| Contributor(s) |
Juelke K, Killig M, Luetke-Eversloh M, Parente E, Grün JR, Morandi B, Ferlazzo G, Thiel A, Schmitt-Knosalla I, Romagnani C |
| Citation(s) |
20505160, 19265543 |
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| Submission date |
May 11, 2010 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Pawel Durek |
| E-mail(s) |
pawel.durek@drfz.de
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| Organization name |
Deutsches Rheuma-Forschungszentrum
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| Street address |
Charitéplatz 1
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| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (9)
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| GSM542578 |
CD62L expression identifies a unique subset of polyfunctional CD56dim NK cells Group1 ND1 |
| GSM542579 |
CD62L expression identifies a unique subset of polyfunctional CD56dim NK cells Group1 ND2 |
| GSM542580 |
CD62L expression identifies a unique subset of polyfunctional CD56dim NK cells Group1 ND3 |
| GSM542581 |
CD62L expression identifies a unique subset of polyfunctional CD56dim NK cells Group2 ND1 |
| GSM542582 |
CD62L expression identifies a unique subset of polyfunctional CD56dim NK cells Group2 ND2 |
| GSM542583 |
CD62L expression identifies a unique subset of polyfunctional CD56dim NK cells Group2 ND3 |
| GSM542584 |
CD62L expression identifies a unique subset of polyfunctional CD56dim NK cells Group3 ND1 |
| GSM542585 |
CD62L expression identifies a unique subset of polyfunctional CD56dim NK cells Group3 ND2 |
| GSM542591 |
CD62L expression identifies a unique subset of polyfunctional CD56dim NK cells Group3 ND3 |
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| Relations |
| BioProject |
PRJNA127297 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE21774_RAW.tar |
41.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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