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| Status |
Public on Apr 20, 2023 |
| Title |
Rio1 downregulates centromeric RNA levels to promote the timely assembly of structurally fit kinetochores |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Kinetochores assemble on centromeres via histone H3 variant CENP-A and low levels of noncoding centromere transcripts (cenRNAs). The latter are ensured by downregulation of RNA polymerase II (RNAPII) and turnover by the nuclear exosome. Using S. cerevisiae, we now add kinase Rio1 to this scheme. Yeast cenRNAs are produced in very low amounts either as short (median lengths of 231nt) or long (4,458nt) transcripts, in a 1:1 ratio. Rio1 limits their production by reducing RNAPII access and transcription activity, and promotes their turnover by the 5’-3’exoribonuclease Rat1. Rio1 similarly curtails the concentrations of noncoding pericenRNAs, which also exist as short transcripts (225nt) at a magnitude higher level than the cenRNAs. In yeast depleted of Rio1, cen- and pericenRNAs accumulatre, and kinetochores misform causing chromosomal instability. The latter phenotypes were also observed with human cells lacking orthologue RioK1, suggesting that CEN regulation by Rio1/RioK1 is evolutionary conserved.
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| |
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| Overall design |
RNA-Seq analysis of RIO1( RIO1-AID) and RIO1 depleted cells (RIO1-AID treated with 500 uM Auxin for 1 hour )
|
| Web link |
https://www.nature.com/articles/s41467-023-38920-9#Sec27
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| |
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| Contributor(s) |
Smurova K, Dassi E, De Wulf P |
| Citation(s) |
37263996 |
| |
| Submission date |
Nov 22, 2022 |
| Last update date |
Jun 06, 2023 |
| Contact name |
Peter De Wulf |
| E-mail(s) |
peter.dewulf@unitn.it
|
| Phone |
(+39)0461285359
|
| Organization name |
University of Trento
|
| Department |
Department of Cellular, Computational and Integrative Biology
|
| Lab |
Chromosome Segregation Biology
|
| Street address |
via Sommarive, 9
|
| City |
Trento |
| State/province |
TN |
| ZIP/Postal code |
38123 |
| Country |
Italy |
| |
|
| Platforms (1) |
| GPL27812 |
Illumina NovaSeq 6000 (Saccharomyces cerevisiae) |
|
| Samples (6)
|
| GSM6753137 |
Rio1 auxin-inducible depletion, 2nd biological repeat |
| GSM6753138 |
RIO1, 3rd biological repeat |
| GSM6753139 |
Rio1 auxin-inducible depletion, 3rd biological repeat |
|
| Relations |
| BioProject |
PRJNA904388 |
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