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| Status |
Public on May 24, 2023 |
| Title |
Dedifferentiation-derived neural stem cells exhibit perturbed temporal progression |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
One of the key regulatory mechanisms of brain size and neuronal diversity is through control of NB identity via cell fate maintenance. Dedifferentiation is the reversion of differentiated cells to a stem cell like fate, whereby, the gene expression program of mature cells is altered and genes associated with multipotency are expressed. Beyond the fact that misexpression of these factors and pathways caused the formation of ectopic NBs, whether these dedifferentiated NBs faithfully produce the correct number and types of neurons or glial cells, or undergo timely terminal differentiation, has not been assessed. These characteristics are key determinants of overall CNS size and function, thus are important parameters when considering whether dedifferentiation leads to tumourigenesis or can be appropriately utilized for regenerative purposes. Appropriate terminal differentiation as well as neuronal diversity are key characteristics of NSCs, and are specified through temporal patterning of the NSCs driven by the successive expression of temporal transcription factors (tTFs). In this study, we found that ectopic NSCs induced via bHLH transcription factor Deadpan (Dpn) expression in the optic lobes of the developing Drosophila CNS fail to undergo appropriate temporal progression, where they express mid-tTF, Sloppy-paired 1 (Slp-1) at the expense of late-tTF Tailless (Tll); consequently generating an excess of Twin of eyeless (Toy) positive neurons and fewer Reversed polarity (Repo) positive glial cells. Dpn overexpression also resulted in stalled progression through the cell cycle, and a failure to undergo timely terminal differentiation. Mechanistically, DamID studies demonstrated that Dpn directly binds to both Dichaete (D), a Sox-box transcription factor known to repress Slp-1, as well as a number of cell cycle genes. Promoting cell cycle progression or overexpression of D were able to re-trigger the progression of the temporal series in dedifferentiated NBs, restoring both neuronal diversity and timely NB terminal differentiation.
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| Overall design |
Targeted DamID-seq (TaDa) for Dpn 3rd instar larval neuroblasts (neural stem cells, NSCs) with Dam-only control; and NanoDam for Dpn in medulla neuroblasts, with corresponding w1118 genetic background control; two replicates per sample.
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| Web link |
https://www.embopress.org/doi/full/10.15252/embr.202255837
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| Contributor(s) |
Marshall OJ, McMullen JP, Cheng LY |
| Citation(s) |
37039033 |
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| Submission date |
Feb 15, 2023 |
| Last update date |
May 24, 2023 |
| Contact name |
Owen Marshall |
| E-mail(s) |
owen.marshall@utas.edu.au
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| Organization name |
Menzies Institute for Medical Research
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| Street address |
17 Liverpool St
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| City |
Hobart |
| State/province |
Tas |
| ZIP/Postal code |
7000 |
| Country |
Australia |
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| Platforms (2) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
| GPL32218 |
DNBSEQ-G400 (Drosophila melanogaster) |
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| Samples (8)
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| GSM7048136 |
Dam-Dpn, larval NSCs, rep2 |
| GSM7048137 |
w1118 genetic background control, NanoDam, larval medulla NSCs, rep1 |
| GSM7048138 |
w1118 genetic background control, NanoDam, larval medulla NSCs, rep2 |
| GSM7048139 |
Dpn-GFP, NanoDam, larval medulla NSCs, rep1 |
| GSM7048140 |
Dpn-GFP, NanoDam, larval medulla NSCs, rep2 |
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| Relations |
| BioProject |
PRJNA935471 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE225409_Dpn-N1-eyGAL4-nanodam-vs-Dam.scaled.gatc.bedgraph.gz |
4.9 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE225409_Dpn-N2-eyGAL4-nanodam-vs-Dam.scaled.gatc.bedgraph.gz |
4.9 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE225409_Dpn-vs-Dam_worGAL4.average-FDR0.01.peaks.gff.gz |
87.1 Kb |
(ftp)(http) |
GFF |
| GSE225409_Dpn-vs-Dam_worGAL4.average.gatc.bedgraph.gz |
5.3 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE225409_Dpn_GFP-vs-Dam_eyGAL4_nanodam.average-FDR0.01.peaks.gff.gz |
70.9 Kb |
(ftp)(http) |
GFF |
| GSE225409_Dpn_GFP-vs-Dam_eyGAL4_nanodam.average.gatc.bedgraph.gz |
5.1 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE225409_Dpn_worG4_N1-vs-Dam.scaled.gatc.bedgraph.gz |
5.3 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE225409_Dpn_worG4_N2-vs-Dam.scaled.gatc.bedgraph.gz |
5.3 Mb |
(ftp)(http) |
BEDGRAPH |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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