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| Status |
Public on Jul 06, 2023 |
| Title |
Identification of an outer membrane protease of Rickettsia rickettsii responsible for maturation of surface exposed autotransporters |
| Organisms |
Rickettsia rickettsii; Chlorocebus aethiops |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Members of the spotted fever group rickettsia express four large, surface-exposed autotransporters, at least one of which is a known virulence determinant. Autotransporter translocation to the bacterial outer surface, also known as type V secretion, involves formation of a b-barrel autotransporter domain in the periplasm that inserts into the outer membrane to form a pore through which the N-terminal passenger domain is passed and exposed on the outer surface. Two major surface antigens of Rickettsia rickettsii, are known to be exposed and the passenger domain cleaved from the autotransporter domain. A highly passaged strain of R. rickettsii, Iowa, fails to cleave these autotransporters and is avirulent. We have identified a putative peptidase, truncated in the Iowa strain, that when reconstituted into Iowa restores appropriate processing of the autotransporters as well as restoring a modest degree of virulence.
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| Overall design |
Comparative gene expression profiling analysis of Rickettsia rickettsii infected Vero cells at 4, 24, 48 hours post infection.
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| Contributor(s) |
Hackstadt T |
| Citation(s) |
37523399 |
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| Submission date |
Mar 07, 2023 |
| Last update date |
Oct 19, 2023 |
| Contact name |
Neelam Redekar |
| Organization name |
NIH
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| Department |
NIAID
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| Street address |
9000 rockville pike
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (2) |
| GPL33219 |
Illumina MiSeq (Chlorocebus aethiops) |
| GPL33220 |
Illumina MiSeq (Rickettsia rickettsii) |
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| Samples (24)
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| GSM7085178 |
Vero76 cells, R. rickettsii st. Morgan, 4hpi, Rep1 |
| GSM7085179 |
Vero76 cells, R. rickettsii st. Morgan, 4hpi, Rep2 |
| GSM7085180 |
Vero76 cells, R. rickettsii st. Morgan, 4hpi, Rep3 |
| GSM7085181 |
Vero76 cells, R. rickettsii st. Iowa, 4hpi, Rep1 |
| GSM7085182 |
Vero76 cells, R. rickettsii st. Iowa, 4hpi, Rep2 |
| GSM7085183 |
Vero76 cells, R. rickettsii st. Iowa, 4hpi, Rep3 |
| GSM7085184 |
Vero76 cells, R. rickettsii st. Morgan, 24hpi, Rep1 |
| GSM7085185 |
Vero76 cells, R. rickettsii st. Morgan, 24hpi, Rep2 |
| GSM7085186 |
Vero76 cells, R. rickettsii st. Morgan, 24hpi, Rep3 |
| GSM7085187 |
Vero76 cells, R. rickettsii st. Iowa, 24hpi, Rep1 |
| GSM7085188 |
Vero76 cells, R. rickettsii st. Iowa, 24hpi, Rep2 |
| GSM7085189 |
Vero76 cells, R. rickettsii st. Iowa, 24hpi, Rep3 |
| GSM7085190 |
Vero76 cells, R. rickettsii st. Morgan, 48hpi, Rep1 |
| GSM7085191 |
Vero76 cells, R. rickettsii st. Morgan, 48hpi, Rep2 |
| GSM7085192 |
Vero76 cells, R. rickettsii st. Morgan, 48hpi, Rep3 |
| GSM7085193 |
Vero76 cells, R. rickettsii st. Iowa, 48hpi, Rep1 |
| GSM7085194 |
Vero76 cells, R. rickettsii st. Iowa, 48hpi, Rep2 |
| GSM7085195 |
Vero76 cells, R. rickettsii st. Iowa, 48hpi, Rep3 |
| GSM7085196 |
Purified R. rickettsii st. Morgan, Rep1 |
| GSM7085197 |
Purified R. rickettsii st. Morgan, Rep2 |
| GSM7085198 |
Purified R. rickettsii st. Morgan, Rep3 |
| GSM7085199 |
Purified R. rickettsii st. Iowa, Rep1 |
| GSM7085200 |
Purified R. rickettsii st. Iowa, Rep2 |
| GSM7085201 |
Purified R. rickettsii st. Iowa, Rep3 |
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| Relations |
| BioProject |
PRJNA941856 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE226832_RSEMcounts_RrIowa_genes.tsv.gz |
61.5 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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