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| Status |
Public on Apr 01, 2024 |
| Title |
Early amyloid-induced changes in microglia gene expression in APP/PS1 mice [bulk RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Alzheimer’s disease (AD) is a progressive neurodegenerative disease and the most common cause of dementia, characterized by deposition of extracellular amyloid-beta (Aβ) aggregates and intraneuronal hyperphosphorylated Tau. Many AD risk genes, identified in genome-wide association studies (GWAS), are expressed in microglia, the innate immune cells of the central nervous system. Specific subtypes of microglia emerged in relation to AD pathology, such as disease-associated microglia (DAMs), which increased in number with age in amyloid mouse models and in human AD cases. However, the initial transcriptional changes in these microglia in response to amyloid are still unknown. Here, to determine early changes in microglia gene expression, hippocampal microglia from APPswe/PS1dE9 (APP/PS1) mice and wildtype littermates were isolated and analyzed by RNA sequencing (RNA-seq). By bulk RNA-seq, transcriptomic changes were detected in hippocampal microglia from 6-months-old APP/PS1 mice. By performing single cell RNA-seq of CD11c-positive and negative microglia from 6-months-old APP/PS1 mice and analysis of the transcriptional trajectory from homeostatic to CD11c-positive microglia, we identified a set of genes that potentally reflect the initial response of microglia to Aβ.
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| Overall design |
Bulk RNA sequencing analysis of FACS-isolated microglia from hippocampus of APPswe/PSEN1dE9 (APP/PS1, transgenic) and controls at different ages ( 4-, 6-, 9-month-old). N = 3 or 4 per condition.
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| Contributor(s) |
Oshima T, Kater MS, Huffels CF, Wesseling EM, Middeldorp J, Hol EM, Verheijen MH, Smit AB, Boddeke EW, Eggen BJ |
| Citation(s) |
38515329 |
| |
| Submission date |
Mar 08, 2023 |
| Last update date |
May 20, 2024 |
| Contact name |
Takuya Oshima |
| E-mail(s) |
t.oshima@umcg.nl
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| Organization name |
University Medical Center Groningen
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| Department |
Department of Biomedical Sciences of Cells & Systems
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| Lab |
Section Molecular Neurobiology
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| Street address |
Antonius Deusinglaan 1
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| City |
Groningen |
| State/province |
Groningen |
| ZIP/Postal code |
9713AV |
| Country |
Netherlands |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE226939 |
Early amyloid-induced changes in microglia gene expression in APP/PS1 mice |
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| Relations |
| BioProject |
PRJNA942288 |
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