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| Status |
Public on Oct 04, 2023 |
| Title |
SIN3 acts in distinct complexes to regulate the germline transcriptional program in C. elegans [sin-3(syb2172) and hda-3(ok1991)] |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The SIN3 transcriptional coregulator influences gene expression through multiple interactions that include histone deacetylases (HDACs). Haploinsufficiency and mutations in SIN3 are the underlying cause of Witteveen-Kolk syndrome and related intellectual disability (ID)/autism syndromes, emphasizing its key role in development. However, little is know on its complexity of interactions and functions in developmental processes. Here we show that loss of SIN-3, the single SIN3 homologue in Caenorhabditis elegans, results in maternal effect sterility associated with deregulation of the germline transcriptome, including derepression of X-linked genes. Using proteomics analysis we identify at least two SIN3 complexes containing distinct HDACs and differentially contributing to fertility. Single cell smFISH reveals that loss of sin-3 stochastically derepresses X linked genes in individual germ cells. We further identify acetylation marks whose abundance depends on SIN3. Together, this work provides a powerful paradigm for the in vivo study of SIN3 and associated proteins.
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| Overall design |
gene expression profiling of Smart-seq data of germlines from young adults sin-3(syb2172) and hda-3(ok1991) versus wild-type (wt)
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| Contributor(s) |
Caron M, Robert V, Gely L, Annie A, Pakulska V, Coute Y, Chevalier M, Riedel C, Bedet C, Palladino F |
| Citation(s) |
37818613, 38746662 |
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| Submission date |
Mar 14, 2023 |
| Last update date |
May 31, 2024 |
| Contact name |
Cecile Bedet |
| E-mail(s) |
cecile.bedet@ens-lyon.fr
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| Organization name |
ENS de Lyon
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| Department |
Bilogie
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| Lab |
LBMC, CNRS UMR5239
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| Street address |
46 allée d'Italie
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| City |
Lyon |
| ZIP/Postal code |
69007 |
| Country |
France |
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| Platforms (1) |
| GPL22765 |
Illumina HiSeq 4000 (Caenorhabditis elegans) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE227499 |
SIN3 acts in distinct complexes to regulate the germline transcriptional program in C. elegans |
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| Relations |
| BioProject |
PRJNA944713 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE227368_RAW.tar |
1.1 Mb |
(http)(custom) |
TAR (of TSV) |
| GSE227368_hda3_vs_wt.csv.gz |
645.1 Kb |
(ftp)(http) |
CSV |
| GSE227368_sin3syb_vs_wt.csv.gz |
660.5 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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