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Series GSE228340 Query DataSets for GSE228340
Status Public on Nov 22, 2023
Title Histone lactylation couples cellular metabolism with the activation of developmental gene regulatory networks [NCC ATAC-seq]
Organism Gallus gallus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Embryonic cells engage in diverse types of metabolism to execute specialized tasks in the developing embryo. Recent studies have demonstrated that metabolic reprogramming can also drive changes in cell identity and behavior by affecting the expression of developmental genes. However, the connection between cellular metabolism and differential gene expression is still not well understood. Here we report found that histone lactylation, an epigenetic mark derived from glycolysis-derived lactate, couples the metabolic state of embryonic cells with gene expression and the activation of gene regulatory networks. Embryonic tissues with high glycolytic flux, like the neural crest and the pre-somitic mesoderm, display high levels of lactylation. The lactylation mark is dynamically deposited in the loci of neural crest genes as these cells transition to a state of enhanced glycolysis. This process promotes accessibility of active enhancers and is necessary for proper deployment of the neural crest gene regulatory network. When we reduced the deposition of the mark by targeting LDHA and LDHB, lactylated genes were downregulated, and neural crest migration was impeded. Lactylation of neural crest enhancers is controlled by transcription factors SOX9 and YAP/TEAD, which are necessary and sufficient for the deposition of the mark. These findings define an epigenetic mechanism that integrates cellular metabolism with the gene regulatory networks that orchestrate embryonic development.
 
Overall design Examination of the accessibility of genomic regions in neural crest cells from HH9 embryos.
 
Contributor(s) Merkuri F, Rothstein M, Simoes-Costa M
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Submission date Mar 27, 2023
Last update date Nov 23, 2023
Contact name Marcos Simoes-Costa
Organization name Boston Children's Hospital
Department Pathology
Street address 300 Longwood Ave
City Boston
State/province Massachusetts
ZIP/Postal code 02115
Country USA
 
Platforms (1)
GPL19787 Illumina NextSeq 500 (Gallus gallus)
Samples (2)
GSM7119209 atac_nf_rep1
GSM7119210 atac_nf_rep2
This SubSeries is part of SuperSeries:
GSE228343 Histone lactylation couples cellular metabolism with the activation of developmental gene regulatory networks
Relations
BioProject PRJNA949372

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Supplementary file Size Download File type/resource
GSE228340_RAW.tar 138.5 Mb (http)(custom) TAR (of BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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