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| Status |
Public on Nov 29, 2011 |
| Title |
Gene expression profiling of Tgfbr2 mutant mouse models of cleft palate |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The overall goal of this project is to investigate the role of TGF-beta signaling in palate development in order to discover candidate therapeutics for preventing and treating congenital birth defects. Here, we conducted gene expression profiling of embryonic palatal tissue from wild type mice as well as those with a neural crest specific conditional inactivation of the Tgfbr2 gene. The latter mice provide a model of cleft palate formation.
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| Overall design |
To investigate the mechanism of cleft palate resulting from mutations in TGFBR2, we analyzed neural crest specific conditional inactivation of Tgfbr2 in mice (Tgfbr2fl/fl;Wnt1-Cre). We performed microarray analyses using the palatal tissue of Tgfbr2fl/fl;Wnt1-Cre mice at embryonic day E13.5 (prior to palatal fusion, n=6 per genotype) and E14.5 (during palatal fusion, n=5 per genotype) to examine the genes regulated by Tgf-beta during palate formation.
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| Contributor(s) |
Iwata J, Hacia JG, Chai Y |
| Citation(s) |
22123828 |
| |
| Submission date |
Jul 19, 2010 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Joseph Gerard Hacia |
| E-mail(s) |
hacia@hsc.usc.edu
|
| Phone |
323-442-3030
|
| Organization name |
University of Southern California
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Hacia Lab
|
| Street address |
2250 Alcazar Street, IGM 261
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90089 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (10)
|
| GSM564967 |
E14.5 Wnt1-Cre;Tgfbr2 Conditional Knockout Mouse Model Palate 1 |
| GSM564968 |
E14.5 Wnt1-Cre;Tgfbr2 Conditional Knockout Mouse Model Palate 2 |
| GSM564969 |
E14.5 Wnt1-Cre;Tgfbr2 Conditional Knockout Mouse Model Palate 3 |
| GSM564970 |
E14.5 Wnt1-Cre;Tgfbr2 Conditional Knockout Mouse Model Palate 4 |
| GSM564971 |
E14.5 Wnt1-Cre;Tgfbr2 Conditional Knockout Mouse Model Palate 5 |
| GSM565084 |
E14.5 Tgfbr2 Wildtype Mouse Model Palate 1 |
| GSM565085 |
E14.5 Tgfbr2 Wildtype Mouse Model Palate 2 |
| GSM565086 |
E14.5 Tgfbr2 Wildtype Mouse Model Palate 3 |
| GSM565087 |
E14.5 Tgfbr2 Wildtype Mouse Model Palate 4 |
| GSM565088 |
E14.5 Tgfbr2 Wildtype Mouse Model Palate 5 |
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| Relations |
| BioProject |
PRJNA127771 |
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