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| Status |
Public on Jun 26, 2023 |
| Title |
Gene expression changes in collagen10a1 (col10a1) medaka mutants |
| Organism |
Oryzias latipes |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To elucidate molecular mechanisms contributing to the disease phenotype in col10a1 medaka (Oryzias latipes) mutants, we isolated col10a1:nlGFP cells from heterozygous col10a1 medaka mutants and wild-type siblings via fluorescence activated cell sorting (FACS) and performed RNA-seq. Comparison of the transcriptome profiles indicated elevated ER stress, dysregulated cell polarity and reduced ECM production in heterozygous col10a1 mutants.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for col10a1:nlGFP-expressing cells in col10a1 medaka mutants and wild-type siblings. Two biological replicates were analyzed for heterozygous col10a1 mutants and three biological replicates were analyzed for wild-type siblings.
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| Contributor(s) |
Tan W, Winkler C |
| Citation(s) |
38510140 |
| |
| Submission date |
May 26, 2023 |
| Last update date |
Apr 02, 2024 |
| Contact name |
Wen Hui Tan |
| E-mail(s) |
tanwenh@gmail.com
|
| Organization name |
National University of Singapore
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| Street address |
14 Science Drive 4
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| City |
Singapore |
| ZIP/Postal code |
117543 |
| Country |
Singapore |
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| Platforms (1) |
| GPL27992 |
Illumina NovaSeq 6000 (Oryzias latipes) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA976693 |
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