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Series GSE23528 Query DataSets for GSE23528
Status Public on Sep 01, 2010
Title Light/dark- and temperature-regulated transcriptional rhythms in adult Caenorhabditis elegans
Organism Caenorhabditis elegans
Experiment type Expression profiling by array
Summary Most organisms have an endogenous circadian clock that is synchronized to environmental signals such as light and temperature. Although circadian rhythms have been described in the nematode C. elegans at the behavioral level, these rhythms appear to be relatively non-robust. Moreover, in contrast to other animal models, no circadian transcriptional rhythms have been identified. Thus, whether this simple nematode contains a bona fide circadian clock remains an open question.
We used microarray experiments to identify light- and temperature-regulated transcriptional rhythms in C. elegans, and show that subsets of these transcripts are regulated in a circadian manner. In addition, we find that light and temperature also globally drive the expression of many genes, indicating that C. elegans exhibits systemic responses to these stimuli.
 
Overall design Populations of growth-synchronized wild-type C. elegans L1 larvae were entrained for 5 days until adulthood to 12:12 hr light/dark (LD) cycles (500-1000 lux) at a constant temperature of 18°C, or for 4 days to 12:12 hr temperature cycles (25:15°C - warm/cold or WC) in constant darkness. RNA was collected every 4 hrs during the last entrainment and the subsequent free-running days and analyzed via hybridization of Affymetrix GeneChips. L4 larvae were transferred to FUDR-containing plates to inhibit embryonic development.
 
Contributor(s) van der Linden AM, Beverly M, Kadener S, Rodriguez J, Wasserman S, Rosbash M, Sengupta P
Citation(s) 20967231
Submission date Aug 09, 2010
Last update date Jul 06, 2016
Contact name Alexander Martinus van der Linden
E-mail(s) avanderlinden@unr.edu
Phone 775-784-6080
Fax 775-784-1302
URL http://www.unr.edu/biology/van%20der%20Linden.htm
Organization name University of Nevada
Department Biology
Lab Van der linden Lab
Street address 1664 N. Virginia St.
City Reno
State/province NV
ZIP/Postal code 89557
Country USA
 
Platforms (1)
GPL200 [Celegans] Affymetrix C. elegans Genome Array
Samples (95)
GSM589315 Adult at T2 in LD, time-series experiment 3
GSM589316 Adult at T6 in LD, time-series experiment 3
GSM589317 Adult at T10 in LD, time-series experiment 3
Relations
BioProject PRJNA131785

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23528_RAW.tar 220.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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