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| Status |
Public on Jun 30, 2023 |
| Title |
Chromatin remodeling events associated with gARID are essential for gametocyte development in Plasmodium [ChIP-seq] |
| Organism |
Plasmodium berghei ANKA |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
To investigate the genome-wide binding sites of gARID in gametocytes of Plasmodium berghei, the chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) analyses were performed. Parasites expressing GFP-fused gARID were harvested after treating the infected mice with sulfadiazine and subjected to ChIP-seq experiments using anti-GFP antibody. Binding sites of gARID were determined from the sequence data.
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| Overall design |
Mice were infected with transgenic parasites and treated with sulfadiazine in their drinking water. Chromatins associated with GFP-tagged gARID were IPed with anti-GFP antibody, and DNA fragments purified from the IPed chromoatins were sequenced by the Next Generation Sequencing. Using non-IPed samples as a control (INPUT), binding sites of gARID were determined from the sequence data.
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| Contributor(s) |
Nishi T, Kaneko I, Iwanaga S, Yuda M |
| Citation missing |
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| |
| Submission date |
Jun 21, 2023 |
| Last update date |
Jul 03, 2023 |
| Contact name |
masao yuda |
| E-mail(s) |
m-yuda@doc.medic.mie-u.ac.jp
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| Organization name |
mie university
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| Street address |
edobashi 2-174
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| City |
tsu |
| State/province |
mie |
| ZIP/Postal code |
514-0001 |
| Country |
Japan |
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| Platforms (1) |
| GPL19670 |
Illumina NextSeq 500 (Plasmodium berghei ANKA) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE235412 |
Chromatin remodeling events associated with gARID are essential for gametocyte development in Plasmodium |
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| Relations |
| BioProject |
PRJNA986006 |
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