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| Status |
Public on Jul 07, 2023 |
| Title |
The subacromial bursa regulates rotator cuff tendon healing and is a target to augment tendon repair. |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Rotator cuff injuries result in over 500,000 surgeries performed annually, an alarmingly high number of which fail. These procedures typically involve repair of the injured tendon and removal of the subacromial bursa. However, recent identification of a resident population of mesenchymal stem cells and inflammatory responsiveness of the bursa to tendinopathy indicate an unexplored biological role of the bursa in the context of rotator cuff disease. Therefore, we aimed to understand the clinical relevance of bursa-tendon crosstalk, characterize the biologic role of the bursa within the shoulder, and test the therapeutic potential for targeting the bursa. Proteomic profiling of patient bursa and tendon samples demonstrated that the bursa is activated by tendon injury. Using a rat to model rotator cuff injury and repair, tenotomy-activated bursa protected the intact tendon adjacent to the injured tendon and maintained the morphology of the underlying bone. The bursa also promoted an early inflammatory response in the injured tendon, initiating key players in wound healing. In vivo results were supported by targeted organ culture studies of the bursa. To examine the potential to therapeutically target the bursa, dexamethasone was delivered to the bursa, prompting a shift in cellular signaling towards modulating inflammation in the healing tendon. In conclusion, contrary to current clinical practice, the bursa should be retained to the greatest extent possible and provides a new therapeutically target for improving tendon healing outcomes.
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| Overall design |
This study identified the role of the subacromial bursa in rotator cuff response to injury via the retention or removal of the subacromial bursa in rotator cuff injury setting. Rat rotator cuff tendon injury (supraspinatus tenotomy) were performed with or without subacromial bursectomy (removal of the subacromial bursa). The resulting bilateral supraspinatus tendons and remaining subacromial bursa were dissected for RNA-sequencing at 3 or 14 days post-operation. There were n=6 replicates per timepoint. Control bursa and supraspinatus tendon from non-operative rats were included for a non-injurious control; supraspinatus tendon samples were also internally controlled per rat.
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| Web link |
https://www.science.org/doi/10.1126/scitranslmed.add8273
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| Contributor(s) |
Marshall BP, Ashinsky BG, Ferrer XE, Kunes JA, Innis AC, Luzzi AJ, Forrester LA, Burt KG, Lee AJ, Song L, Lisiewski LE, Soon RK, Hung CT, Levine WN, Kovacevic D, Thomopoulos S |
| Citation(s) |
38657023 |
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| Submission date |
Jun 26, 2023 |
| Last update date |
Apr 24, 2024 |
| Contact name |
Stavros Thomopoulos |
| E-mail(s) |
sat2@columbia.edu
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| Organization name |
Columbia University
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| Lab |
Carroll Laboratories for Orthopedic Surgery
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| Street address |
650 W 168 ST
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
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| Platforms (1) |
| GPL25947 |
Illumina NovaSeq 6000 (Rattus norvegicus) |
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| Samples (46)
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| GSM7510850 |
Bursa Control, rep 4 |
| GSM7510851 |
Bursa Control, rep 5 |
| GSM7510852 |
Bursa day 14 Activated, rep 1 |
| GSM7510853 |
Bursa day 14 Activated, rep 2 |
| GSM7510854 |
Bursa day 14 Activated, rep 3 |
| GSM7510855 |
Bursa day 14 Activated, rep 4 |
| GSM7510856 |
Bursa day 14 Activated rep 5 |
| GSM7510857 |
Bursa day 14 Activated, rep 6 |
| GSM7510858 |
Bursa day 3 Activated, rep 1 |
| GSM7510859 |
Bursa day 3 Activated, rep 2 |
| GSM7510860 |
Bursa day 3 Activated, rep 3 |
| GSM7510861 |
Bursa day 3 Activated, rep 4 |
| GSM7510862 |
Bursa day 3 Activated, rep 5 |
| GSM7510863 |
Bursa day 3 Activated, rep 6 |
| GSM7510864 |
Supraspinatus Control, rep 1 |
| GSM7510865 |
Supraspinatus Control, rep 2 |
| GSM7510866 |
Supraspinatus Control, rep 3 |
| GSM7510867 |
Supraspinatus Control, rep 4 |
| GSM7510868 |
Supraspinatus Control, rep 5 |
| GSM7510869 |
Supraspinatus day 14 Bursa removed, rep 1 |
| GSM7510870 |
Supraspinatus day 14 Bursa removed, rep 2 |
| GSM7510871 |
Supraspinatus day 14 Bursa removed, rep 3 |
| GSM7510872 |
Supraspinatus day 14 Bursa removed, rep 4 |
| GSM7510873 |
Supraspinatus day 14 Bursa removed, rep 5 |
| GSM7510874 |
Supraspinatus day 14 Bursa removed, rep 6 |
| GSM7510875 |
Supraspinatus day 14 Bursa intact, rep 1 |
| GSM7510876 |
Supraspinatus day 14 Bursa intact, rep 2 |
| GSM7510877 |
Supraspinatus day 14 Bursa intact, rep 3 |
| GSM7510878 |
Supraspinatus day 14 Bursa intact, rep 4 |
| GSM7510879 |
Supraspinatus day 14 Bursa intact, rep 5 |
| GSM7510880 |
Supraspinatus day 14 Bursa intact, rep 6 |
| GSM7510881 |
Supraspinatus day 3 Bursa removed, rep 1 |
| GSM7510882 |
Supraspinatus day 3 Bursa removed, rep 2 |
| GSM7510883 |
Supraspinatus day 3 Bursa removed, rep 3 |
| GSM7510884 |
Supraspinatus day 3 Bursa removed, rep 4 |
| GSM7510885 |
Supraspinatus day 3 Bursa removed, rep 5 |
| GSM7510886 |
Supraspinatus day 3 Bursa removed, rep 6 |
| GSM7510887 |
Supraspinatus day 3 Bursa intact, rep 1 |
| GSM7510888 |
Supraspinatus day 3 Bursa intact, rep 2 |
| GSM7510889 |
Supraspinatus day 3 Bursa intact, rep 3 |
| GSM7510890 |
Supraspinatus day 3 Bursa intact, rep 4 |
| GSM7510891 |
Supraspinatus day 3 Bursa intact, rep 5 |
| GSM7510892 |
Supraspinatus day 3 Bursa intact, rep 6 |
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| Relations |
| BioProject |
PRJNA987719 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE235861_DESeq2_kallisto_SB011_SB014_SB017_SB020_SB023_SB026_vs_SB002_SB004_SB006_SB008_SB010-DEG_list.csv.gz |
981.1 Kb |
(ftp)(http) |
CSV |
| GSE235861_DESeq2_kallisto_SB011_SB014_SB017_SB020_SB023_SB026_vs_SB013_SB016_SB019_SB022_SB025_SB028-DEG_list.csv.gz |
872.0 Kb |
(ftp)(http) |
CSV |
| GSE235861_DESeq2_kallisto_SB012_SB015_SB018_SB021_SB024_SB027_vs_SB001_SB003_SB005_SB007_SB009-DEG_list.csv.gz |
972.2 Kb |
(ftp)(http) |
CSV |
| GSE235861_DESeq2_kallisto_SB013_SB016_SB019_SB022_SB025_SB028_vs_SB002_SB004_SB006_SB008_SB010-DEG_list.csv.gz |
980.0 Kb |
(ftp)(http) |
CSV |
| GSE235861_DESeq2_kallisto_SB029_SB032_SB035_SB038_SB041_SB044_vs_SB002_SB004_SB006_SB008_SB010-DEG_list.csv.gz |
978.1 Kb |
(ftp)(http) |
CSV |
| GSE235861_DESeq2_kallisto_SB029_SB032_SB035_SB038_SB041_SB044_vs_SB011_SB014_SB017_SB020_SB023_SB026-DEG_list.csv.gz |
974.1 Kb |
(ftp)(http) |
CSV |
| GSE235861_DESeq2_kallisto_SB029_SB032_SB035_SB038_SB041_SB044_vs_SB031_SB034_SB037_SB040_SB043_SB046-DEG_list.csv.gz |
892.5 Kb |
(ftp)(http) |
CSV |
| GSE235861_DESeq2_kallisto_SB030_SB033_SB036_SB039_SB042_SB045_vs_SB001_SB003_SB005_SB007_SB009-DEG_list.csv.gz |
982.1 Kb |
(ftp)(http) |
CSV |
| GSE235861_DESeq2_kallisto_SB030_SB033_SB036_SB039_SB042_SB045_vs_SB012_SB015_SB018_SB021_SB024_SB027-DEG_list.csv.gz |
992.1 Kb |
(ftp)(http) |
CSV |
| GSE235861_DESeq2_kallisto_SB031_SB034_SB037_SB040_SB043_SB046_vs_SB002_SB004_SB006_SB008_SB010-DEG_list.csv.gz |
983.7 Kb |
(ftp)(http) |
CSV |
| GSE235861_DESeq2_kallisto_SB031_SB034_SB037_SB040_SB043_SB046_vs_SB013_SB016_SB019_SB022_SB025_SB028-DEG_list.csv.gz |
974.7 Kb |
(ftp)(http) |
CSV |
| GSE235861_est_counts_transcipts_kallisto.txt.gz |
1.6 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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