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| Status |
Public on Jul 31, 2024 |
| Title |
Prtg+ve Rhombic Lip Ventricular Zone Stem Cells Initiate and Maintain Group 3 Medulloblastoma [human_tumours_bulk] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Total RNA sequencing was performed to understand the transcriptome alterations in Gr3-medulloblastoma lines induced by the indirect interactions of endothelial cells.
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| |
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| Overall design |
Briefly, Group 3 medulloblastoma PDX (Med-411FH, Med-211FH) and cell lines (D425, MB002) were cocultured on a porous membrane insert (0.4µm) along with HUVEC (Human umbilical vein endothelial cells). The membrane allows the exchange of secreted factors. For the control condition, Medulloblastoma cells were plated alone on the insert. Post 72hrs of incubation, the cells were collected for RNA isolation.
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| |
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| Contributor(s) |
Taylor MD |
| Citation(s) |
38971152 |
| |
| Submission date |
Jul 14, 2023 |
| Last update date |
Jul 31, 2024 |
| Contact name |
Vikas Patil |
| Organization name |
Princess Margaret Cancer Center
|
| Lab |
MacFeeters-Hamilton Centre for Neuro-Oncology Research
|
| Street address |
610 University Ave.
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M3A3B3 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE237381 |
Prtg+ve Rhombic Lip Ventricular Zone Stem Cells Initiate and Maintain Group 3 Medulloblastoma |
|
| Relations |
| BioProject |
PRJNA994850 |
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