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| Status |
Public on Oct 04, 2023 |
| Title |
Increased pro-inflammatory phenotype of peripheral blood monocytes and monocyte-derived macrophages among patients with excessive ethanol intake |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Merkel cell polyomavirus; Mus musculus cytomegalovirus 2; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Excessive alcohol consumption adversely affects the immune system and triggers the activation of peripheral blood (PB) monocytes and tissue macrophages, contributing to alcohol- related organ damage. This study aimed to analyze the M1/M2 and inflammatory phenotypes of circulating monocytes and macrophage-derived monocytes (MDMs). This single-center cross- sectional study included 20 excessive alcohol drinkers (EADs) and 22 healthy controls. PB samples were collected under fasting conditions for isolation of CD14+ monocytes and short-term culture without stimulation, LPS/IFNγ, or IL4/IL13. These conditions were also used to polarize monocyte-derived macrophages (MDMs) into M0, M1, or M2 phenotypes. Cytokine production was assessed in the blood samples and supernatants.
M1/M2 related markers in PB monocytes and MDMs were analyzed using mRNA expression and surface marker analyses. In addition, the miRNA profile was analyzed in CD14+ monocytes. PB samples from EADs exhibited increased levels of pro-inflammatory cytokines.
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| Overall design |
In the present study, 22 samples are included, of which 8 samples are control samples from peripheral blood CD14+ monocytes, healthy controls, and 14 samples from CD14+ monocytes from patients with excessive alcohol intake.
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| Contributor(s) |
Fernández-Regueras M, Carbonell C, Salete-Granado D, García J, Gragera-Cabezudo M, Pérez-Nieto M, Mayado A, Torres J, Usategui-Martín R, Rojas-Pirela M, Sabio G, Orfao A, González-Sarmiento R, Laso FJ, Almeida J, Marcos M |
| Citation(s) |
37760011 |
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| Submission date |
Jul 31, 2023 |
| Last update date |
Oct 05, 2023 |
| Contact name |
Juan Luis Garcia |
| E-mail(s) |
jlgarcia@usal.es
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| Phone |
923294812
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| Organization name |
CIC/IBSAL
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| Street address |
Campus Miguel de Unamuno Centro de Investigacion del Cancer, IBSAL
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| City |
Salamanca |
| State/province |
Type Below |
| ZIP/Postal code |
37007 |
| Country |
Spain |
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| Platforms (1) |
| GPL11434 |
miRCURY LNA microRNA Array, 6th generation - hsa, mmu & rno |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA1000493 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE239659_RAW.tar |
18.3 Mb |
(http)(custom) |
TAR (of GPR, TXT) |
| Processed data included within Sample table |
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