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| Status |
Public on Aug 21, 2011 |
| Title |
The inflammasome is a central player in the induction of obesity and insulin resistance |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Inflammation plays a key role in the pathogenesis of obesity. Chronic overfeeding leads to macrophage infiltration in the adipose tissue, resulting in pro-inflammatory cytokine production. Both microbial and endogenous danger signals trigger assembly of the intracellular innate immune sensor Nlrp3 [NLR family, pyrin domain containing 3] resulting in caspase-1 activation and production of pro-inflammatory cytokines interleukin (IL)-1beta and IL-18. Here, we showed that mice deficient in Nlrp3, ASC [apoptosis-associated speck-like protein containing a CARD; a.k.a PYCARD (PYD and CARD domain containing)] and caspase-1 were resistant to the development of high fat diet-induced obesity, which correlated with protection from obesity-induced insulin resistance. Detailed metabolic and molecular phenotyping demonstrated that the inflammasome controls energy expenditure and adipogenic gene expression during chronic overfeeding. These findings reveal a critical function of the inflammasome in obesity and insulin resistance and suggest inhibition of the inflammasome as a potential therapeutic strategy.
Keywords: Expression profiling by array
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| Overall design |
Wild-type (WT), ASC-null and Casp1-null mice were subjected to high fat diet feeding for 16 weeks. After the diet intervention period, the animals were killed and epididymal white adipose tissue was removed. Total RNA was isolated and subjected to gene expression profiling.
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| Web link |
http://humannutrition2.wur.nl/stienstra2011/
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| Contributor(s) |
Stienstra R, van Diepen JA, Tack CJ, Zaki MH, van de Veerdonk FL, Perera D, Neal G, Hijmans A, Vroegrijk IO, van den Berg SA, Romijn JA, Rensen PC, Joosten LA, Netea MG, Kanneganti TD |
| Citation(s) |
21876127 |
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| Submission date |
Nov 08, 2010 |
| Last update date |
Aug 06, 2018 |
| Contact name |
Guido Hooiveld |
| E-mail(s) |
guido.hooiveld@wur.nl
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| Organization name |
Wageningen University
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| Department |
Div. Human Nutrition & Health
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| Lab |
Nutrition, Metabolism & Genomics Group
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| Street address |
HELIX, Stippeneng 4
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| City |
Wageningen |
| ZIP/Postal code |
NL-6708WE |
| Country |
Netherlands |
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| Platforms (1) |
| GPL11180 |
[HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate |
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| Samples (10)
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| GSM619226 |
Epidydimal white adipose tissue from Casp1-/- after 16 weeks of high fat diet feeding, replicate 1 |
| GSM619227 |
Epidydimal white adipose tissue from Casp1-/- after 16 weeks of high fat diet feeding, replicate 2 |
| GSM619228 |
Epidydimal white adipose tissue from Casp1-/- after 16 weeks of high fat diet feeding, replicate 3 |
| GSM619229 |
Epidydimal white adipose tissue from WT after 16 weeks of high fat diet feeding, replicate 1 |
| GSM619230 |
Epidydimal white adipose tissue from WT after 16 weeks of high fat diet feeding, replicate 2 |
| GSM619231 |
Epidydimal white adipose tissue from WT after 16 weeks of high fat diet feeding, replicate 3 |
| GSM619232 |
Epidydimal white adipose tissue from WT after 16 weeks of high fat diet feeding, replicate 4 |
| GSM619233 |
Epidydimal white adipose tissue from ASC-/- after 16 weeks of high fat diet feeding, replicate 1 |
| GSM619234 |
Epidydimal white adipose tissue from ASC-/- after 16 weeks of high fat diet feeding, replicate 2 |
| GSM619235 |
Epidydimal white adipose tissue from ASC-/- after 16 weeks of high fat diet feeding, replicate 3 |
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| Relations |
| BioProject |
PRJNA134319 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE25205_RAW.tar |
21.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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