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| Status |
Public on Feb 20, 2024 |
| Title |
Analysis of the stress response after induction of the dormancy-inducing membrane toxin TisB in Escherichia coli |
| Organism |
Escherichia coli |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We assume that adaptation to tisB expression is driven by cellular stress responses. It has already been observed that expression of type I toxins leads to up-regulation of several stress-related genes. To reveal the response to TisB on a global scale, transcriptome analysis of MG1655 p0SD-tisB was performed.
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| Overall design |
Wild type MG1655, harboring the p0SD-tisB plasmid, was treated with L-ara for tisB induction during exponential phase for 30 min.
RNA samples of biological triplicates were extracted before (exp) and after L-ara treatment (0ATG), and after 1 hour (rec1h) and 2 hours (rec2h) of recovery.
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| Contributor(s) |
Leinberger FH, Edelmann D, Schmid NE, Blumenkamp P, Berghoff BA |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Feb 14, 2024 |
| Last update date |
Feb 20, 2024 |
| Contact name |
Bork A. Berghoff |
| Organization name |
University of Giessen
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| Street address |
Heinrich-Buff-Ring 26-32
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| City |
Giessen |
| ZIP/Postal code |
35392 |
| Country |
Germany |
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| Platforms (1) |
| GPL21222 |
Illumina NextSeq 500 (Escherichia coli) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA1076485 |
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