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| Status |
Public on Jun 21, 2024 |
| Title |
An isogenic E. coli population gives rise to multiple persister phenotypes |
| Organism |
Escherichia coli |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Antibiotic treatment typically eliminates a significant portion of a bacterial population, leaving behind a smaller subset of tolerant cells that can survive the treatment. These tolerant cells hinder the effectiveness of the antibiotic, potentially leading to the development of antibiotic resistance within the population. Antibiotic tolerance differs from resistance: tolerant cells are unable to grow or reproduce in the presence of the antibiotic, but they can proliferate once the antibiotic is removed. However, in cases of resistance, the antibiotic loses its efficacy entirely, posing a significant threat to public health. Our study challenges the long-held consensus that persisters are completely dormant and are of one single population. Our results clearly show that persisters are not as dormant as once thought, and multiple populations of persisters form during lethal antibiotic treatment despite the cells being genetically identical. We compared the transcriptome profiles at different time points to investigate the dynamic changes and/or existence of multiple persister subpopulations in response to lethal antibiotic ampicillin (Amp) and ciprofloxacin (Cip) treatment in E. coli.
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| Overall design |
Stationary phase cells were diluted 1:100 times in pre-warmed MMB+ media and treated with Amp (0.1 mg/mL) for 24 h, and samples were isolated for RNA sequencing before antibiotic treatment (0 h) and after antibiotic treatment at 3 h, 6 h, and 24 h.
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| Contributor(s) |
Hossain T, Rahman K, Amaratunga R, Butzin NC, Singh A |
| Citation missing |
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| BioProject |
PRJNA1067386 |
| |
| Submission date |
Feb 20, 2024 |
| Last update date |
Jun 21, 2024 |
| Contact name |
Nicholas C Butzin |
| E-mail(s) |
nicholas.butzin@gmail.com
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| Phone |
6056884078
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| Organization name |
South Dakota State University
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| Department |
Biology and Microbiology
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| Lab |
Butzin Lab
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| Street address |
1224 Medary Ave.
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| City |
Brookings |
| State/province |
SD |
| ZIP/Postal code |
57007 |
| Country |
USA |
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| Platforms (1) |
| GPL14548 |
Illumina HiSeq 2000 (Escherichia coli) |
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| Samples (6)
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