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| Status |
Public on Oct 11, 2011 |
| Title |
Expression data from Caco-2 cells expressing TLR4 and associated mutants |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Common missense mutations (D299G, T399I) have been recently identified in the human TLR4 gene. The aim of this study was to determine how TLR4 and associated mutants affect gene expression in Caco-2 cells.
We used microarrays to asses gene expression profiles in Caco-2 stably overexpressing TLR4-WT, TLR4-D299G, TLR4-T399I or untransfected.
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| Overall design |
Caco-2 clones stably overexpressing HA-tagged wildtype TLR4-WT, mutant TLR4-D299G or TLR4-T399I were generated. Prior to analysis, cell clones were cultured for 8 days in all experiments. RNA (triplicate) was extracted and hybridized on Affymetrix microarrays.
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| Contributor(s) |
Cario E, Eyking A |
| Citation(s) |
21920464 |
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| Submission date |
Dec 21, 2010 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Elke Cario |
| E-mail(s) |
elke.cario@uni-due.de
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| Phone |
+49-201-723-4527
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| Organization name |
University of Duisburg-Essen, University Hospital of Essen
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| Department |
Division of Gastroenterology and Hepatology
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| Lab |
Cario
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| Street address |
Virchowstr. 171
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| City |
Essen |
| State/province |
NRW |
| ZIP/Postal code |
45147 |
| Country |
Germany |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA134989 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE26226_RAW.tar |
51.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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