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| Status |
Public on Apr 24, 2024 |
| Title |
Identification of cambium stem cell factors and their positioning mechanism |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Wood constitutes the majority of terrestrial biomass.Composed of xylem, it arises from one side of the vascular cambium, a bifacial stem cell niche that also produces phloem on the opposing side. It is currently unknown which molecular factors endowcambium stem cell identity. Here we show that TDIF ligand-activated PXY receptors promote the expression of CAMBIUM AINTEGUMENTA-LIKE (CAIL) transcription factors to define cambium stem cell identity inthe Arabidopsisroot. By sequestrating the phloem-originated TDIF, xylem-dependent PXY confines the TDIF signaling front, resulting in the activation of CAIL expression and stem cell identity in only a narrow domain. Our findings show how signals emanating from cells on opposing sides ensure robust yet dynamically adjustable positioning of a bifacial stem cell.
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| Overall design |
There are 2 groups (Mock, Induced), made up of 3 biological replicates with 2 time points (8hours, 24hours) totalling 12 samples. For each sample, 1.5cm of root segments below the root-hypocotyl junction were collected from 15 individuals, and visible lateral roots were removed.
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| Contributor(s) |
Eswaran G, Zhang J, Blomster T, Mähönen AP |
| Citation missing |
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| BioProject |
PRJNA1101261 |
| Submission date |
Apr 19, 2024 |
| Last update date |
Apr 24, 2024 |
| Contact name |
Xixi Zhang |
| E-mail(s) |
xixi.zhang@helsinki.fi
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| Phone |
0401852968
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| Organization name |
University of Helsinki
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| Street address |
Pläsinpellonkuja 4 B 50
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| City |
Helsinki |
| ZIP/Postal code |
00740 |
| Country |
Finland |
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| Platforms (1) |
| GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
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| Samples (12)
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| GSM8217652 |
Arabidopsis, Induced, 8 hours, rep 1 |
| GSM8217654 |
Arabidopsis, Induced, 8 hours, rep 2 |
| GSM8217655 |
Arabidopsis, Induced, 8 hours, rep 3 |
| GSM8217656 |
Arabidopsis, Mock, 24 hours, rep 1 |
| GSM8217657 |
Arabidopsis, Mock, 24 hours, rep 2 |
| GSM8217658 |
Arabidopsis, Mock, 24 hours, rep 3 |
| GSM8217659 |
Arabidopsis, Induced, 24 hours, rep 1 |
| GSM8217661 |
Arabidopsis, Induced, 24 hours, rep 2 |
| GSM8217662 |
Arabidopsis, Induced, 24 hours, rep 3 |
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| Supplementary file |
Size |
Download |
File type/resource |
| GSE264403_RAW.tar |
5.9 Mb |
(http)(custom) |
TAR (of TSV) |
SRA Run Selector |
| Raw data are available in SRA |
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