 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 20, 2024 |
| Title |
CRISPR/Cas9 regulates EphA2-CDH1 axis to inhibit the migration of castration-resistant prostate cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We ued CRISPR/Cas9 to knock out EphA2 gene in PC3 cells, and overexpress EphA2 gene in Du145 cells with overexpressed plasmids to explore the underlying mechanisms of EphA2 function in castration-resistant prostate cancer(CRPC) and clinical relevance, providing more evidence for EphA2 as a potential therapeutic target for CRPC.
|
| |
|
| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for PC3-EphA2-KO and control cells, DU145-EphA2-OE and control cells.
|
| |
|
| Contributor(s) |
Yan S, Li X, Chen Y, Pan P, Hu N, Wei L, Xiao Y, Xiao L, Wei C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 15, 2024 |
| Last update date |
May 20, 2024 |
| Contact name |
Chaogang Wei |
| E-mail(s) |
20214133003@stu.suda.edu.cn
|
| Organization name |
The Second Affiliated Hospital of Soochow University
|
| Street address |
1055 Sanxiang Road, Suzhou 215004, Jiangsu, China
|
| City |
Suzhou |
| ZIP/Postal code |
215000 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (8)
|
| GSM8267483 |
PC3 cells,EphA2 gene knock out(infected with the CRISPR/Cas9 lentiviral vectors), Rep1 |
| GSM8267484 |
PC3 cells,EphA2 gene knock out(infected with the CRISPR/Cas9 lentiviral vectors), Rep2 |
| GSM8267485 |
PC3 cells,infected with the CRISPR/Cas9 control lentiviral vectors, Rep1 |
| GSM8267486 |
PC3 cells,infected with the CRISPR/Cas9 control lentiviral vectors, Rep2 |
| GSM8267487 |
DU145 cells,EphA2 gene overexpressed(transfected with the EphA2 expression plasmids), Rep1 |
| GSM8267488 |
DU145 cells,EphA2 gene overexpressed(transfected with the EphA2 expression plasmids), Rep2 |
| GSM8267489 |
DU145 cells,transfected with the control plasmids, Rep1 |
| GSM8267490 |
DU145 cells,transfected with the control plasmids, Rep2 |
|
| Relations |
| BioProject |
PRJNA1111754 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE267508_4_genes_fpkm_expression.xlsx |
9.5 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
|
|
|
|
 |
Less...
More...