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| Status |
Public on Jun 01, 2024 |
| Title |
Fosl2 orchestrates chromatin accessibility dynamics to determine mammalian ovarian folliculogenesis [ATAC-seq] |
| Organisms |
Sus scrofa; Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
In mammalian ovaries, granulosa cells (GCs) behave as the most active cell lineage in terms of differentiation and proliferation, ensuring precise developmental gene expression pattern and enhancing continuous ovarian folliculogenesis; however, how developing GCs is interplay with downstream transcriptional activation in mammals remain poorly understood. In this research, we depicted the genome-wide redistribution of accessible chromatin regions that lead to broad developmental-related transcriptome effects spanning pig and mouse follicular stages. We report the distinct GCs-activated accessibility regions (GAAs) at MII stage are always distributed on H3K4me3-anchored promoter regions, where they are responsible for elevated flanking development related genes (DRGs) expression, serving as a key requirement for transcription in response to developmental signals. Mechanistically, the transcription factor, Fosl2, can be mightily recruited to GAAs to accomplish chromatin accessibility state transition. The enlarged GAAs signal driven by Fosl2 loading subsequently induces a robust increase in the expression of adjacent DRGs expression, which contribute to the progression of ovarian folliculogenesis. Together, our study delineates the dynamic chromatin accessibility landscape across various stages of folliculogenesis, uncovering a pivotal role for the Fosl2 in controlling the transcriptional activation through the reconfiguration of the widespread chromatin state.
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| Overall design |
Paired-end ATAC-seq of oocytes and GCs in pig and mouse to sought the accessible chromatin spanning follicular stages.
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| Contributor(s) |
Guan Y |
| Citation missing |
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| |
| Submission date |
May 20, 2024 |
| Last update date |
Jun 01, 2024 |
| Contact name |
Yiting Guan |
| E-mail(s) |
yt_guan@yeah.net
|
| Organization name |
Central People's Hospital of Zhanjiang
|
| Department |
Zhanjiang Institute of Clinical Medicine
|
| Street address |
236 Yuanzhu Road
|
| City |
Zhanjiang |
| ZIP/Postal code |
524045 |
| Country |
China |
| |
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| Platforms (2) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL26351 |
Illumina NovaSeq 6000 (Sus scrofa) |
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| Samples (19)
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| This SubSeries is part of SuperSeries: |
| GSE267974 |
Fosl2 orchestrates chromatin accessibility dynamics to determine mammalian ovarian folliculogenesis. |
|
| Relations |
| BioProject |
PRJNA1113521 |
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