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| Status |
Public on May 09, 2011 |
| Title |
Expression data from MCF-7 cells stimulated by Estrogen or IGF-I |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Although estrogen receptor (ER) and insulin-like growth factor (IGF) signaling are important for normal mammary development and breast cancer, cross-talk between these pathways, particularly at the level of gene transcription, remains poorly understood. We performed microarray analysis on MCF-7 breast cancer cells treated with estradiol (E2) or IGF-I for 3hr or 24hr. IGF-I regulated mRNA of 5-10-fold more genes than estradiol, and many genes were co-regulated by both ligands. Importantly, expression of these co-regulated genes correlated with poor prognosis of human breast cancer. Closer examination revealed enrichment of repressed transcripts. Interestingly, a number of potential tumor suppressors were down-regulated by IGF-I and estradiol. In fact, BLNK, one of the top repressed genes, is a potential growth suppressor in breast cancer cells. Analysis of three down-regulated genes showed that E2-mediated repression occurred independently of IGF-IR, and IGF-I-mediated repression occurred independently of ER. However, repression by IGF-I or estradiol required common downstream kinases. In conclusion, E2 and IGF-I co-regulate a set of genes that affect breast cancer outcome. There is enrichment of repressed transcripts, and the down-regulation is independent at the receptor level. This may be important clinically, as tumors with active ER and IGF-IR signaling may require co-targeting of both pathways.
KEYWORDS: multiple group comparison
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| Overall design |
Microarray analysis on MCF-7 breast cancer cells treated with estradiol (E2) or IGF-I for 3hr or 24hr.
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| Contributor(s) |
Creighton C, Tsimelzon A, Casa A, Lee A |
| Citation(s) |
21541704 |
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| Submission date |
Jan 24, 2011 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Chad Creighton |
| E-mail(s) |
creighto@bcm.tmc.edu
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| Organization name |
Baylor College of Medicine
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| Department |
Biostatistics, Ducan Cancer Center
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| Street address |
One Baylor Plaza, Mail Stop: BCM305
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (17)
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| GSM659805 |
SFM control cells (3hr), biological rep_1 |
| GSM659806 |
SFM control cells (3hr), biological rep_2 |
| GSM659807 |
E2-treated cells (3hr), biological rep_1 |
| GSM659808 |
E2-treated cells (3hr), biological rep_2 |
| GSM659809 |
E2-treated cells (3hr), biological rep_3 |
| GSM659810 |
IGF-treated cells (3hr), biological rep_1 |
| GSM659811 |
IGF-treated cells (3hr), biological rep_2 |
| GSM659812 |
IGF-treated cells (3hr), biological rep_3 |
| GSM659813 |
SFM control cells (24hr), biological rep_1 |
| GSM659814 |
SFM control cells (24hr), biological rep_2 |
| GSM659815 |
SFM control cells (24hr), biological rep_3 |
| GSM659816 |
E2-treated cells (24hr), biological rep_1 |
| GSM659817 |
E2-treated cells (24hr), biological rep_2 |
| GSM659818 |
E2-treated cells (24hr), biological rep_3 |
| GSM659819 |
IGF-treated cells (24hr), biological rep_1 |
| GSM659820 |
IGF-treated cells (24hr), biological rep_2 |
| GSM659821 |
IGF-treated cells (24hr), biological rep_3 |
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| Relations |
| BioProject |
PRJNA136041 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE26834_RAW.tar |
54.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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