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| Status |
Public on Jun 10, 2024 |
| Title |
Prediction of Non-Coding sRNAs and Screening of Virulence-Related sRNAs of Salmonella Pullorum |
| Organism |
Salmonella enterica subsp. enterica serovar Pullorum |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Purpose: Searching for sRNAs in Salmonella pullorum by RNA sequencing and exploring their functions.Methods: High-throughput sequencing of RNA extracted from Salmonella pullorum under normal growth conditions to detect newly discovered sRNAs, followed by experiments to verify their functions.Results: The proportion of Clean Reads of this sequencing was >65%, and the base Q30s were all above 85%, indicating that the sequencing quality is good and can be used for subsequent analysis. The sRNAscanner software predicted that 148 new sRNAs might exist on the reference genome of Salmonella fowl dysentery, and the reads obtained from sequencing were compared to the genome, and it was found that 110 out of the 148 newly predicted sRNAs could be detected.Conclusions: sRNAs are widely found in bacteria and are involved in many physiological processes. In this study, we detected new sRNAs in Salmonella pullorum by RNA-seq, which lays the foundation for the subsequent investigation of the regulatory functions of sRNAs in bacteria.
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| Overall design |
Salmonella pullorum were cultured to logarithmic growth in LB medium, total RNA was extracted ，in triplicate, and 150 bp bipartite sequencing was performed using an Illumina NovaSeq 6000 high-throughput sequencer.
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| Contributor(s) |
He T, Li T |
| Citation missing |
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| Submission date |
Jun 05, 2024 |
| Last update date |
Jun 10, 2024 |
| Contact name |
HE Ting |
| E-mail(s) |
ht1723595275@outlook.com
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| Phone |
15708925598
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| Organization name |
Hainan University
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| Street address |
Hainan University, Meilan District, Haikou City, Hainan Province, China
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| City |
Haikou |
| ZIP/Postal code |
570228 |
| Country |
China |
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| Platforms (1) |
| GPL34564 |
Illumina NovaSeq 6000 (Salmonella enterica subsp. enterica serovar Pullorum) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA1120424 |
