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| Status |
Public on Aug 05, 2005 |
| Title |
TGF-beta regulated gene transcription and protein expression in human GFAP-negative lamina cribrosa cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Primary open angle glaucoma (POAG) is a progressive optic neuropathy, which is a major cause of worldwide visual impairment and blindness. Pathological hallmarks of the glaucomatous optic nerve head include retinal ganglion cell axon loss and extracellular matrix (ECM) remodeling of the lamina cribrosa layer. TGF-beta is an important pro-fibrotic modulator of ECM metabolism, whose levels are elevated in human POAG lamina cribrosa tissue compared with non-glaucomatous controls. We treated human lamina cribrosa (LC) cells with TGF-beta1 (10ng/ml) for 24 hours in order to examine differential gene expression patterns in repsonse to this cytokine. In particular we focused on ECM and fibrotic genes. We found that TGF-beta1 induces expression and release of ECM components in LC cells, which may be important in regulating matrix remodeling in the lamina cribrosa. In disease states such as POAG, the LC cell may represent an important pro-fibrotic cell type and an attractive target for novel therapeutic strategies.
Keywords: other
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| Contributor(s) |
Kirwan RP, Leonard MO, Murphy M, Clark AF, O'Brien CJ |
| Citation(s) |
16078232 |
| |
| Submission date |
May 24, 2005 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Ruaidhri Patrick Kirwan |
| E-mail(s) |
ruaidhri.kirwan@ucd.ie
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| Organization name |
University College Dublin
|
| Department |
Conway Institute of Biomolecular and Biomedical Research & School of Medicine and Medical Science
|
| Lab |
Dr. Martin Leonard
|
| Street address |
Belfield
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| City |
Dublin |
| State/province |
Leinster |
| ZIP/Postal code |
D4 |
| Country |
Ireland |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (4)
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| GSM52088 |
TGFb1 treated experiment A (10ng/ml 24 hours) |
| GSM52089 |
vehicle treated experiment A (4mM HCL/1% BSA) 24 hours |
| GSM52090 |
TGFb1 treated experiment B (10ng/ml 24 hours) |
| GSM52091 |
vehicle treated experiment B (4mM HCL/1% BSA) 24 hours |
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| Relations |
| BioProject |
PRJNA92353 |
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