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| Status |
Public on Jun 24, 2024 |
| Title |
Activation of the PARIS immune complex by viral proteins results in host tRNA cleavage and can be overcome by viruses encoding non-cleavable tRNAs |
| Organism |
Escherichia coli |
| Experiment type |
Other
|
| Summary |
Viruses compete with other viruses for limited cellular recourses, and some viruses deliver defense mechanisms that protect the host from competing genetic parasites. PARIS is a defense system, often encoded in viral genomes, that is composed of a 53 kDa ABC ATPase (AriA) and a 35 kDa TOPRIM nuclease (AriB). Here we show that AriA and AriB assemble into a 425 kDa supramolecular immune complex. We use cryo-EM to determine the structure of this complex which explains how six molecules of AriA assemble into a propeller-shaped scaffold that coordinates three subunits of AriB. ATP-dependent detection of foreign proteins triggers the release of AriB, which assembles into a homodimeric nuclease that blocks infection by cleaving the host Lysine tRNA. Phage T5 can subvert PARIS immunity through expression of a non-cleavable tRNA variant. Collectively, these data explain how AriA functions as an ATP-dependent sensor that detects viral proteins and activates the AriB toxin. PARIS is one of an emerging set of immune systems that form macromolecular complexes for the recognition of foreign proteins, rather than foreign nucleic acids.
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| Overall design |
to study the chromosome organization in Paris conditoin we used HiC sample
|
| Web link |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10802454/
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| |
|
| Contributor(s) |
Groseille J, Bikard D, Wiedenheft B, Isaev A |
| Citation(s) |
39111359 |
| |
| Submission date |
Jun 24, 2024 |
| Last update date |
Sep 23, 2024 |
| Contact name |
Justine Rozenn Groseille |
| E-mail(s) |
justine.groseille@pasteur.fr
|
| Organization name |
Institut Pasteur
|
| Street address |
28 rue du Dr Roux
|
| City |
Paris |
| ZIP/Postal code |
75015 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL21222 |
Illumina NextSeq 500 (Escherichia coli) |
|
| Samples (6)
|
| GSM8346094 |
Hi-C of E.coli: MG1655 + DPAG 50 uM (15 min) WT |
| GSM8346095 |
Hi-C of E.coli :MG1655 + DPAG 50 uM (30 min) WT |
| GSM8346096 |
Hi-C of E.coli :MG1655 + DPAG 50 uM (15min) Paris |
| GSM8346097 |
Hi-C of E.coli :MG1655 + DPAG 50 uM (30 min) Paris |
| GSM8346098 |
Hi-C of E.coli :MG1655 + cm 20 µg/ml Contrôle (15min) |
| GSM8346099 |
Hi-C of E.coli :MG1655 + cm 20 µg/ml Contrôle (30min) |
|
| Relations |
| BioProject |
PRJNA1127446 |
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