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| Status |
Public on Jul 10, 2024 |
| Title |
Effect of dopamine on gene expression in SK-N-SH cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Dopaminylation, the covalent attachment of dopamine to the side chain of glutamine (Gln, Q) in proteins, has been identified as a class of posttranslational modification. Due to the limited number of substrates, the functions and underlying molecular mechanisms of dopaminylation are not fully characterized. Utilizing an alkyne-functionalized dopamine probe, we have developed a method to selectively enrich dopaminylated proteins in the whole-cell context. We identified 4133 proteins potentially modified with dopamine and validated the modification of histone H4 glutamine 27 by dopamine (H4Q27dop). H4Q27dop can inhibit cell proliferation through downregulating cyclin D1 gene CCND1 transcription, a classical well-known proliferation promoter. Our study provides a valuable resource of putative substrate proteins modified with dopamine and reveals a novel mechanism of dopamine regulating cell growth in a neuroblastoma cancer model.
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| Overall design |
Comparative ChIP-Seq analysis of H4Q27dop in SK-N-SH cells under control conditions and with dopamine treatment.
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| Contributor(s) |
Zhang Y, Yang Y, Wu W, Song W |
| Citation missing |
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| |
| Submission date |
Jul 03, 2024 |
| Last update date |
Jul 10, 2024 |
| Contact name |
Yang Yaqi |
| E-mail(s) |
Yyaqi111@hotmail.com
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| Phone |
13952272870
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| Organization name |
Shanghai Children Medical Centre
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| Street address |
No.1678 Dongfang Road, Pudong, Shanghai, China
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| City |
Shanghai |
| ZIP/Postal code |
200127 |
| Country |
China |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA1131463 |
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