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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 29, 2011 |
| Title |
T cell dependent immune responses: B cell activation and differentiation Group1 Fo, Group2 GC1, Group3 GC2 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Upon immunization with a T cell dependent antigen naive follicular B cells (Fo) are activated and a germinal center reaction is induced. Within the next 2 weeks large germinal centers develop where the process of affinity maturation takes place. To analyze the gene expression profile of resting and activated B cells, follicular B cells (Fo), B cells from early (GC1) and late germinal centers (GC2) were isolated and their gene expression profile compared.
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| Overall design |
Gene expression profiles of Fo versus GC1 and GC2 B cells, respectively. Naïve Fo B cells were isolated from non-immunized BALB/c mice. Germinal center B cells sorted from spleen cell suspensions of BALB/-c mice immunized with the T cell dependent antigen 2-phenyl Oxazolone. GC1 B cells were isolated 7 days after primary immunization. GC2 cells were isolated 15 days after primary immunization.
After total RNA extraction, reverse transcription, cDNA extraction, the biotinylated cRNA was transcribed, fragmented, and 15 µg cRNA hybridized in duplicates for each of the three groups to the GeneChip arrays. Group1: Fo, Group2: GC1, Group3: GC2. Lists of differentially regulated genes were created using High Performance Chip Data Analysis (HPCDA) with Bioretis database (http://www.bioretis-analysis.de). Worldwide data sharing is possible via Bioretis, please ask the authors.
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| Contributor(s) |
Wilke G, Steinhauser G, Grün JR, Berek C |
| Citation(s) |
20518031, 19265543 |
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| Submission date |
Mar 29, 2011 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Pawel Durek |
| E-mail(s) |
pawel.durek@drfz.de
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| Organization name |
Deutsches Rheuma-Forschungszentrum
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| Street address |
Charitéplatz 1
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| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| GSM699068 |
T cell dependent immune responses: B cell activation and differentiation Group1 follicular B cells (Fo), Chip 2 |
| GSM699069 |
T cell dependent immune responses: B cell activation and differentiation Group1 follicular B cells (Fo), Chip 3 |
| GSM699070 |
T cell dependent immune responses: B cell activation and differentiation Group2 early germinal center Bcells (GC1) Chip1 |
| GSM699071 |
T cell dependent immune responses: B cell activation and differentiation Group2 early germinal center Bcells (GC1) Chip2 |
| GSM699072 |
T cell dependent immune responses: B cell activation and differentiation Group3 late germinal center B cell (GC2) Chip1 |
| GSM699073 |
T cell dependent immune responses: B cell activation and differentiation Group3 late germinal center B cell (GC2) Chip2 |
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| Relations |
| BioProject |
PRJNA139581 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE28237_RAW.tar |
24.9 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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