 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jun 01, 2011 |
| Title |
Microarray analysis of the astrocyte transcriptome in the ageing brain: relationship to Alzheimer's pathology and ApoE genotype |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Astrocyte dysfunction impacts their normal function, including neuronal support, thereby contributing to neurodegenerative pathologies including Alzheimer's disease (AD). Therefore to understand the role of astrocytes in the pathogenesis of age-related disorders we analysed the gene expression profile of astrocytes with respect to Alzheimer-type pathology.
The aim of the present study was to combine immuno-LCM and microarray analysis to characterise the astrocyte transcriptome at different Braak stages, and with respect to ApoE genotype, in post-mortem human temporal cortex sampled dervied from the Medical Research Council Cognitive Function and Ageing Study (MRC-CFAS).
|
| |
|
| Overall design |
GFAP-positive astrocytes were isolated from age, sex and brain pH-matched cases derived from the MRC-CFAS cohort, using immuno laser capture microdissection. The extracted RNA was amplified and applied to HGU133 Plus 2.0 Affymetrix gene arrays. Genes were considered differentially expressed if they showed a minimum 1.5 fold change at p<0.02 in all 6 individual cases in each stage of pathology [18 cases in total: 6 cases Braak stages I-II (3 cases carried at least one ApoE epsilon4 allele and 3 were ApoEe4 negative); 6 cases Braak stages III-IV (3 ApoEe4 positive and 3 ApoEe4 negative; 6 cases Braak stages V-VI (3 ApoEe4 positive and 3 ApoEe4 negative)].
keywords: human GFAP-positive astrocytes
|
| |
|
| Contributor(s) |
Simpson JE, Ince PG, Shaw PJ, Heath PR, Raman R, Garwood CJ, Gelsthorpe C, Baxter L, Forster G, Matthews FE, Brayne C, Wharton SB |
| Citation(s) |
21705112 |
| |
| Submission date |
Jun 01, 2011 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Paul Roy Heath |
| E-mail(s) |
p.heath@sheffield.ac.uk
|
| Phone |
+44 114 2222254
|
| Organization name |
University of Sheffield
|
| Department |
Academic Neurology Unit
|
| Lab |
Heath
|
| Street address |
SITraN, 385a Glossop Road
|
| City |
Sheffield |
| State/province |
S. Yorks |
| ZIP/Postal code |
S10 2HQ |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (18)
|
| GSM735094 |
Braak I-II, APOE e4+ temporal cortex astrocytes |
| GSM735095 |
Braak I-II, APOE e4+ sample 2 |
| GSM735096 |
Braak I-II, APOE e4+ sample 3 |
| GSM735097 |
Braak I-II, APOE e4- temporal cortex astrocytes |
| GSM735098 |
Braak I-II, APOE e4- sample 2 |
| GSM735099 |
Braak I-II, APOE e4- sample 3 |
| GSM735100 |
Braak III-IV, APOE e4+ temporal cortex astrocytes |
| GSM735101 |
Braak III-IV, APOE e4+ sample 2 |
| GSM735102 |
Braak III-IV, APOE e4+ sample 3 |
| GSM735103 |
Braak III-IV, APOE e4- temporal cortex astrocytes |
| GSM735104 |
Braak III-IV, APOE e4- sample 2 |
| GSM735105 |
Braak III-IV, APOE e4- sample 3 |
| GSM735106 |
Braak V-VI, APOE e4+ temporal cortex astrocytes |
| GSM735107 |
Braak V-VI, APOE e4+ sample 2 |
| GSM735108 |
Braak V-VI, APOE e4+ sample 3 |
| GSM735109 |
Braak V-VI, APOE e4- sample 1 |
| GSM735110 |
Braak V-VI, APOE e4- sample 2 |
| GSM735111 |
Braak V-VI, APOE e4- sample 3 |
|
| Relations |
| BioProject |
PRJNA141241 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE29652_RAW.tar |
64.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...