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Status |
Public on Jul 20, 2011 |
Title |
Deregulation of Gene Expression induced by Environmental Tobacco Smoke Exposure in Pregnancy |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Passive smoke intake by pregnant women may have detrimental effects such as spontaneous abortion, lower birth weight, stillbirth, and reduced infant lung function. To extend our knowledge on molecular effects of tobacco smoke exposure in pregnancy, we analyzed transcriptome alterations in passive smokers (PS) and compared them to those in active smokers (AS). Using Illumina Expression Beadchip with 24,526 transcript probes, gene expression patterns were assayed in placentas from PS (N=25) exposed to environmental tobacco smoke (ETS) throughout pregnancy and non-exposed (NS) counterparts (N=35), and in cord blood cells from their newborns. The ETS exposure was evaluated by questionnaire disclosure and cotinine measurement in maternal and cord bloods. A total of 196 genes were significantly deregulated in placentas of PS compared to NS. These genes were primary associated with extracellular matrix, apoptosis, blood clotting, response to stress, embryonic morphogenesis, and lipid metabolism. Cord blood of newborns of PS displayed differential expression of 116 genes encoding mainly neuronal factors, regulators of immunologic response, and protooncogenes. Gene ontology analyses highlighted some important biological processes that might be associated with placental insufficiency and fetal growth restriction in PS, such as fatty acid catabolism, coagulation, regulation of growth, and response to steroid hormone stimulus. The study demonstrates that even low dose exposure to ETS during pregnancy leads to the significant deregulation of transcriptional regulation in placental and fetal cells. The data suggest the effect of ETS on the fetus is primary indirect, mediated via deregulation of placental functions. Comparison of PS and AS indicated that ETS exposure and active smoking in pregnancy partly employ the same molecular mechanisms.
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Overall design |
A total of 60 women who gave birth to a baby in the Faculty Hospital Motol (Czech Republic) were enrolled into the study approved by the local Institutional Review Board. The participants signed written informed consent and self-reported information on tobacco smoke exposure (type of exposure, number of cigarettes per day, exposure period) and life style during pregnancy (alcohol drinking, diet, area of residency etc.). Further, the women completed the questionnaire on pregnancy/delivery course (complications, diseases, medication, and delivery mode) and newborn characteristics (physical parameters, Apgar score) with the assistance of gynecologist. Only women without any health complications during pregnancy and their newborns without manifestation of pathological abnormalities were included in the study. Based on self-reported tobacco smoke exposure, the women were divided into two groups, non-smokers (NS, N=35) and passive smokers (PS, N=25). Former and active smokers were excluded from the study. Exposure to tobacco smoke was further evaluated by measurement of plasma cotinine levels in maternal peripheral blood and newborn cord blood using radioimmunoassay.
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Contributor(s) |
Votavova H, Merkerova-Dostalova M, Krejcik Z, Fejglova K, Vasikova A, Tabashidze N, Pastorkova A, Topinka J, Balascak I, Sram RJ, Brdicka R |
Citation(s) |
22355075 |
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Submission date |
Jun 16, 2011 |
Last update date |
Mar 20, 2017 |
Contact name |
Hana Bruchova |
E-mail(s) |
Hana.Bruchova@uhkt.cz
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Phone |
+420221977306
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Fax |
+420221977371
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Organization name |
Institute of Hematology and Transfusion
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Department |
Molecular Genetics
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Lab |
Genomics
|
Street address |
U nemocnice 1
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City |
Prague 2 |
ZIP/Postal code |
128 20 |
Country |
Czech Republic |
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Platforms (1) |
GPL6883 |
Illumina HumanRef-8 v3.0 expression beadchip |
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Samples (104)
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GSM743219 |
non-smoker 205, placenta |
GSM743220 |
passive smoker 206, placenta |
GSM743221 |
passive smoker 207, placenta |
GSM743222 |
non-smoker 208, placenta |
GSM743223 |
passive smoker 209, placenta |
GSM743224 |
non-smoker 210, placenta |
GSM743225 |
non-smoker 211, placenta |
GSM743226 |
passive smoker 212, placenta |
GSM743227 |
passive smoker 215, placenta |
GSM743228 |
non-smoker 218, placenta |
GSM743229 |
passive smoker 219, placenta |
GSM743230 |
non-smoker 220, placenta |
GSM743231 |
non-smoker 222, placenta |
GSM743232 |
passive smoker 225, placenta |
GSM743233 |
non-smoker 226, placenta |
GSM743234 |
non-smoker 228, placenta |
GSM743235 |
non-smoker 231, placenta |
GSM743236 |
passive smoker 232, placenta |
GSM743237 |
passive smoker 234, placenta |
GSM743238 |
non-smoker 236, placenta |
GSM743239 |
non-smoker 238, placenta |
GSM743240 |
non-smoker 240, placenta |
GSM743241 |
non-smoker 241, placenta |
GSM743242 |
passive smoker 245, placenta |
GSM743243 |
passive smoker 246, placenta |
GSM743244 |
non-smoker 248, placenta |
GSM743245 |
passive smoker 251, placenta |
GSM743246 |
passive smoker 256, placenta |
GSM743247 |
passive smoker 258, placenta |
GSM743248 |
non-smoker 259, placenta |
GSM743249 |
non-smoker 260, placenta |
GSM743250 |
non-smoker 261, placenta |
GSM743251 |
passive smoker 262, placenta |
GSM743252 |
passive smoker 263, placenta |
GSM743253 |
non-smoker 265, placenta |
GSM743254 |
non-smoker 266, placenta |
GSM743255 |
passive smoker 267, placenta |
GSM743256 |
non-smoker 268, placenta |
GSM743257 |
passive smoker 270, placenta |
GSM743258 |
passive smoker 274, placenta |
GSM743259 |
non-smoker 275, placenta |
GSM743260 |
non-smoker 282, placenta |
GSM743261 |
non-smoker 284, placenta |
GSM743262 |
non-smoker 287, placenta |
GSM743263 |
passive smoker 289, placenta |
GSM743264 |
non-smoker 291, placenta |
GSM743265 |
non-smoker 292, placenta |
GSM743266 |
non-smoker 294, placenta |
GSM743267 |
passive smoker 295, placenta |
GSM743268 |
non-smoker 296, placenta |
GSM743269 |
non-smoker 297, placenta |
GSM743270 |
non-smoker 298, placenta |
GSM743271 |
non-smoker 299, placenta |
GSM743272 |
non-smoker 300, placenta |
GSM743273 |
passive smoker 201, cord blood |
GSM743274 |
passive smoker 203, cord blood |
GSM743275 |
non-smoker 204, cord blood |
GSM743276 |
passive smoker 209, cord blood |
GSM743277 |
non-smoker 210, cord blood |
GSM743278 |
non-smoker 211, cord blood |
GSM743279 |
passive smoker 212, cord blood |
GSM743280 |
passive smoker 215, cord blood |
GSM743281 |
non-smoker 218, cord blood |
GSM743282 |
passive smoker 219, cord blood |
GSM743283 |
non-smoker 222, cord blood |
GSM743284 |
passive smoker 225, cord blood |
GSM743285 |
non-smoker 226, cord blood |
GSM743286 |
non-smoker 228, cord blood |
GSM743287 |
non-smoker 231, cord blood |
GSM743288 |
passive smoker 232, cord blood |
GSM743289 |
passive smoker 234, cord blood |
GSM743290 |
non-smoker 236, cord blood |
GSM743291 |
non-smoker 238, cord blood |
GSM743292 |
non-smoker 240, cord blood |
GSM743293 |
non-smoker 241, cord blood |
GSM743294 |
passive smoker 245, cord blood |
GSM743295 |
passive smoker 246, cord blood |
GSM743296 |
non-smoker 248, cord blood |
GSM743297 |
passive smoker 251, cord blood |
GSM743298 |
non-smoker 252, cord blood |
GSM743299 |
passive smoker 253, cord blood |
GSM743300 |
passive smoker 256, cord blood |
GSM743301 |
passive smoker 258, cord blood |
GSM743302 |
passive smoker 262, cord blood |
GSM743303 |
passive smoker 263, cord blood |
GSM743304 |
non-smoker 265, cord blood |
GSM743305 |
non-smoker 266, cord blood |
GSM743306 |
passive smoker 267, cord blood |
GSM743307 |
non-smoker 268, cord blood |
GSM743308 |
passive smoker 270, cord blood |
GSM743309 |
passive smoker 274, cord blood |
GSM743310 |
non-smoker 284, cord blood |
GSM743311 |
non-smoker 287, cord blood |
GSM743312 |
passive smoker 289, cord blood |
GSM743313 |
non-smoker 291, cord blood |
GSM743314 |
non-smoker 292, cord blood |
GSM743315 |
passive smoker 293, cord blood |
GSM743316 |
non-smoker 294, cord blood |
GSM743317 |
passive smoker 295, cord blood |
GSM743318 |
non-smoker 299, cord blood |
GSM743319 |
non-smoker 300, cord blood |
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Relations |
BioProject |
PRJNA144057 |
Supplementary file |
Size |
Download |
File type/resource |
GSE30032_RAW.tar |
3.9 Mb |
(http)(custom) |
TAR |
GSE30032_non-normalized_cord-blood.txt.gz |
5.8 Mb |
(ftp)(http) |
TXT |
GSE30032_non-normalized_placenta.txt.gz |
7.1 Mb |
(ftp)(http) |
TXT |
Processed data included within Sample table |
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