|
Status |
Public on Dec 24, 2011 |
Title |
Integration of BRCA1-mediated miRNA and mRNA signatures reveal miR-146a, miR-99b and miR-205 regulation of the TRAF2 and NFkB pathways (miRNA dataset) |
Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Betapolyomavirus hominis; Betapolyomavirus macacae |
Sample organism |
Homo sapiens |
Experiment type |
Non-coding RNA profiling by array
|
Summary |
Microarray‐based techniques are being used to obtain miRNA and gene expression signatures associated with different samples. In order to deepen our understanding of BRCA1-associated tumorigenesis, we integrated data from microarray experiments to obtain significant miRNA-mRNA relationships associated with the presence of the BRCA1 gene. We obtained significant miRNA-gene-pathway relationships underlying the array signatures. Furthermore, we have demonstrated that miR-146a, miR-99b and miR-205, induced in HCC1937 BRCA1-expressing cells, commonly regulate the TRAF2 gene, a key regulator of NF-κB and MAPK pathways. In addition, re-expression of miR-146a, miR-99b or miR-205 in HCC1937 BRCA1-null cells was sufficient to modulate NF-κB activity. Thus, integration between miRNA-mRNA expression data allowed us to define genes and pathways controlled by miRNAs induced in the context of BRCA1 expression.
|
|
|
Overall design |
Comparison of miRNA expression profiles between two isogenic cell lines differing in BRCA1 gene expression status. Single-color experiments in a pairwise comparison design with three technical replicates per cell line.
|
|
|
Contributor(s) |
Tanic M, Zajac M, Gomez-Lopez G, Benitez J, Martinez B |
Citation(s) |
22167321 |
|
Submission date |
Jul 18, 2011 |
Last update date |
May 10, 2016 |
Contact name |
Beatriz Martinez |
E-mail(s) |
bmartinez@cnio.es
|
Organization name |
Spanish National Cancer Research Centre (CNIO)
|
Department |
Human Cancer Genetics
|
Street address |
Calle Melchor Fernandez Almagro 3
|
City |
Madrid |
ZIP/Postal code |
28029 |
Country |
Spain |
|
|
Platforms (1) |
GPL7723 |
miRCURY LNA microRNA Array, v.11.0 - hsa, mmu & rno |
|
Samples (6)
|
|
This SubSeries is part of SuperSeries: |
GSE30822 |
Integration of BRCA1-mediated miRNA and mRNA signatures reveal miR-146a, miR-99b and miR-205 regulation of the TRAF2 and NFkB pathways |
|
Relations |
BioProject |
PRJNA154713 |