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Status |
Public on Dec 24, 2011 |
Title |
Integration of BRCA1-mediated miRNA and mRNA signatures reveal miR-146a, miR-99b and miR-205 regulation of the TRAF2 and NFkB pathways (mRNA dataset) |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
BRCA1 deregulation is a frequent event in the pathogenesis of breast as well as other cancers. In addition to the DNA repair functions of BRCA1, it is involved in a wide range of cellular processes such as cell cycle, chromatin remodeling or transcription. However, the molecular events underlying BRCA1-associated tumorigenesis are still largely unknown. In order to deepen our understanding of BRCA1-associated tumorigenesis, we integrated data from mRNA and miRNA microarray experiments on the HCC1937 breast cancer cell line, and the isogenic HCC1937 stably expressing BRCA1, to obtain significant miRNA-mRNA relationships associated to the presence of the BRCA1 gene. Our results demonstrate that integration of mRNA and miRNA associated to BRCA1 expression was useful to discover new miRNA-gene interactions as molecular events underlying BRCA1-mediated tumorigenesis.
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Overall design |
Transcriptional profiling of the BRCA1-null HCC1937 cell line and HCC1937 cells after stable transfection of BRCA1. Two-condition experiment: Universal Human Reference RNA (Stratagene, catalog #740000) (Cy3) vs. cell line (Cy5). Biological replicates: 3 HCC1937, 3 BRCA1-transfected HCC1937 cells.
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Contributor(s) |
Tanic M, Zajac M, Gómez-López G, Benítez J, Martínez-Delgado B |
Citation(s) |
22167321 |
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Submission date |
Jul 20, 2011 |
Last update date |
Jan 23, 2019 |
Contact name |
Beatriz Martinez |
E-mail(s) |
bmartinez@cnio.es
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Organization name |
Spanish National Cancer Research Centre (CNIO)
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Department |
Human Cancer Genetics
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Street address |
Calle Melchor Fernandez Almagro 3
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City |
Madrid |
ZIP/Postal code |
28029 |
Country |
Spain |
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Platforms (1) |
GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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Samples (6)
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This SubSeries is part of SuperSeries: |
GSE30822 |
Integration of BRCA1-mediated miRNA and mRNA signatures reveal miR-146a, miR-99b and miR-205 regulation of the TRAF2 and NFkB pathways |
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Relations |
BioProject |
PRJNA154567 |