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| Status |
Public on May 10, 2012 |
| Title |
Knock-down and Over-expression of JMJD6 in MCF-7 and/or MDA-MB231 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
By survival analysis of breast cancer patients, JMJD6 was found to be significantly associated with poor prognosis. Over-expression and knock-down of JMJD6 in breast cancer cell lines suggested a role in proliferation. In order to study the transcriptional events that occur following JMJD6 expression changes, siRNA-mediated knock-down of JMJD6 was performed in MCF-7 and MDA-MB231 and stable over-expression of JMJD6 was performed in MCF-7.
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| |
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| Overall design |
There are 2 different siRNA-mediated knock-downs of JMJD6 with 2 biological replicates in MCF-7 and MDA-MB231; 3 clones of JMJD6 over-expression with 3 biological replicates in MCF-7. The control for the knock-downs is scrambled siRNA-treated MCF-7 and MDA-MB231 and the control for JMJD6 over-expression is empty vector over-expression in MCF-7.
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| |
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| Contributor(s) |
Lee Y, Desai KV |
| Citation(s) |
22621393 |
| |
| Submission date |
Aug 31, 2011 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Yi Fang Lee |
| E-mail(s) |
leeyf1@gis.a-star.edu.sg
|
| Organization name |
Genome Institute of Singapore
|
| Street address |
60 Biopolis Street
|
| City |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (28)
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| Relations |
| BioProject |
PRJNA145293 |
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