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| Status |
Public on Nov 06, 2012 |
| Title |
Transcriptome analysis of mediator Med20 mutant and dcr1 mutant in S.pombe |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Expression profiling by genome tiling array
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| Summary |
At Schizosaccharomyces pombe centromeres, non-coding RNAs are transcribed by RNA polymerase II and processed by the RNAi machinery to direct heterochromatin formation. Mediator is a regulator of RNA polymerase II transcription and we here demonstrated that it also controls the levels of centromeric transcripts. Loss of the Mediator subunit Med20 increases transcription of non-coding RNAs and disrupts the centromeric heterochromatin structure.
We have used tilling arrays to study the gene expression changes in Med20 deletionVs WT and Med20 dcr1 double mutant Vs Dcr1 mutant in exponentially growing fission yeast cells to detect how the Med20 gene affect centromeric expression.
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| Overall design |
RNA samples were taken from exponentially growing fission yeast cells and reverse transcribed cDNA from the RNA are hybridized to affymetrix pombe tilling arrays: wild type,med20 mutant, Dcr1 mutants and med20,dcr1 double mutant.
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| Contributor(s) |
Carlsten J, Zhu X |
| Citation(s) |
22851695 |
| |
| Submission date |
Sep 22, 2011 |
| Last update date |
May 27, 2014 |
| Contact name |
Claes M Gustafsson |
| E-mail(s) |
claes.gustafsson@medkem.gu.se
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| Organization name |
Gothenburg University
|
| Department |
Department of Medical biochemistry and cell biology
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| Street address |
Medcinaregatan 9A
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| City |
Gothenburg |
| ZIP/Postal code |
405 30 |
| Country |
Sweden |
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| Platforms (1) |
| GPL7715 |
[Sp20b_M] Affymetrix S. pombe Tiling 1.0FR Array |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE35527 |
Mediator promotes CENP-A incorporation at fission yeast centromeres |
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| Relations |
| BioProject |
PRJNA155507 |
