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| Status |
Public on Nov 30, 2011 |
| Title |
A single intradermal injection of IFN-γ induces a psoriasis-like state in both non-lesional psoriatic and healthy skin |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Psoriasis is a chronic, debilitating, immune-mediated inflammatory skin disease. As IFN-γ is involved in many cellular processes, including activation of T cells and dendritic cells (DCs), antigen processing and presentation, cell adhesion and trafficking, and cytokine and chemokine production, IFN-γ-producing Th1 cells were proposed to be integral to the pathogenesis of psoriasis. Recently, IFN-γ was shown to enhance IL-23 and IL-1 production by DCs and subsequently induce Th17 cells, important contributors to the inflammatory cascade in psoriasis lesions. To determine if IFN-γ indeed induces the pathways leading to the development of psoriasis lesions, a single intradermal injection of IFN-γ was administered to an area of clinically normal, non-lesional skin of psoriasis patients and biopsies were collected 24 hours later. Although there were no visible changes in the skin, IFN-γ induced molecular and histological features characteristic of psoriasis lesions. IFN-γ increased a number of differentially expressed genes in the skin, including many chemokines concomitant with an influx of T cells and inflammatory DCs. Furthermore, inflammatory DC products TNF, iNOS, IL-23, and TRAIL were present in IFN-γ-treated skin. Thus, IFN-γ, which is significantly elevated in non-lesional skin compared to healthy skin, appears to be a key pathogenic cytokine that can induce the inflammatory cascade in psoriasis.
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| Overall design |
RNA was isolated from whole skin punch biopsies of either healthy or non-lesional psoraisis patients at baseline or 24 hours after placebo or IFN-g injection.
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| Contributor(s) |
Suárez-Fariñas M, Johnson-Huang LM, Lowes MA |
| Citation(s) |
22277938 |
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| Submission date |
Sep 27, 2011 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Mayte Suarez-Farinas |
| E-mail(s) |
mayte.suarezfarinas@mssm.edu
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| Organization name |
Mount SinaiSchool of Medicine
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| Street address |
1425 Madison Ave, L2-70C, Box 1077,
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10075 |
| Country |
USA |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (60)
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| Relations |
| BioProject |
PRJNA146401 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE32407_RAW.tar |
129.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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