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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 01, 2011 |
| Title |
Gene expression profiles of AML derived stem cells: similarity to hematopoietic stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Tumors contain a fraction of cancer stem cells that maintain the propagation of the disease. The CD34CD38_ cells, isolated from acute myeloid leukemia (AML), were shown to be enriched leukemic stem cells (LSC). We isolated the CD34CD38_ cell fraction from AML and compared their gene expression profiles to the CD34CD38 cell fraction, using microarrays. We found 409 genes that were at least twofold over- or underexpressed between the two cell populations. These include underexpression of DNA repair, signal transduction and cell cycle genes, consistent with the relative quiescence of stem cells, and chromosomal aberrations and mutations of leukemic cells. Comparison of the LSC expression data to that of normal hematopoietic stem cells (HSC) revealed that 34% of the modulated genes are shared by both LSC and HSC, supporting the suggestion that the LSC originated within the HSC progenitors. We focused on the Notch pathway since Jagged-2, a Notch ligand was found to be overexpressed in the LSC samples. We show that DAPT, an inhibitor of gamma-secretase, a protease that is involved in Jagged and Notch signaling, inhibits LSC growth in colony formation assays. Identification of additional genes that regulate LSC self-renewal may provide new targets for therapy.
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| Overall design |
Microarrays were used to compare the gene expression patterns between AML CD34+CD38- cells and AML CD34+CD38+
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| Contributor(s) |
Gal H, Amariglio N, Trakhtenbrot L, Jacob-Hirsch J, Margalit O, Avigdor A, Nagler A, Tavor S, Ein-Dor L, Lapidot T, Domani E, Rechavi G, Givol D |
| Citation(s) |
17039238 |
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| Submission date |
Nov 30, 2011 |
| Last update date |
Oct 25, 2022 |
| Contact name |
jasmine Jacob |
| E-mail(s) |
j-jacob@sheba.health.gov.il
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| Phone |
0523790500
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| Organization name |
Sheba Medical Center at Tel HaShomer
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| Street address |
haChevel 33
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| City |
Shoham |
| State/province |
-Select- |
| ZIP/Postal code |
60850 |
| Country |
Israel |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA148063 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE34044_RAW.tar |
14.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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