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| Status |
Public on Nov 01, 2005 |
| Title |
Guided differentiation of mouse embryonic stem cell |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The mouse embryonic stem cell’s differentiation was guided by several treatments, and each stage of differentiation was examined.
Keywords: development stage
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| Overall design |
EBs were dissociated and plated onto tissue culture dishes in the serum-free medium containing Insulin/Transferrin/Selenium/Fibronectin (ITSF). After 6 days, the cells were expanded and plated onto polyornithine (15 mg ml-1) and laminin-coated plates (Becton Dickinson Labware, Bedford, MA) with the N2 medium (Johe, 1996) supplemented with laminin, bFGF (R&D Systems, Minneapolis, MN), murine N-terminal fragment of SHH and murine FGF8 isoform b (R&D Systems) (stage 4) . Finally the cells were induced to differentiated into DA neurons by removal of bFGF. The differentiation medium consisted of N2 medium supplemented with laminin in the presence or absence of cAMP, AA (Sigma, St. Louis, MO). The cells were incubated under differentiation conditions for 6–15 days.
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| Contributor(s) |
Park W, Jun D, Hwang C |
| Citation(s) |
16627687 |
| |
| Submission date |
Oct 28, 2005 |
| Last update date |
Jul 26, 2013 |
| Contact name |
Woong Yang Park |
| E-mail(s) |
wypark@snu.ac.kr
|
| Phone |
+82-02-740-8241
|
| Fax |
+82-02-744-4534
|
| Organization name |
Seoul National University
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Molecular and Genomic Medicine
|
| Street address |
28 Yongondong, Chongnogu
|
| City |
Seoul |
| ZIP/Postal code |
110-799 |
| Country |
South Korea |
| |
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| Platforms (1) |
| GPL2987 |
Macrogen MAC Mouse 11K Oligo Microarray |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE5459 |
Selection of Neural Differentiation-Specific Genes by Comparing Profiles of Random Differentiation |
|
| Relations |
| BioProject |
PRJNA104825 |
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