|
| Status |
Public on Mar 21, 2013 |
| Title |
Comparison of bone-marrow mesenchymal stromal cells from multiple myeloma patients and healthy donors |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
It is now well established that bone marrow (BM) constitutes a microenvironment required for differentiation. Bone marrow mesenchymal stromal cells (BM-MSCs) strongly support MM cell growth, by producing a high level of Interleukin-6 (IL-6), a major MM cell growth factor. BM-MSCs also support osteoclastogenesis and angiogenesis. Previous studies have suggested that the direct (VLA-4, VCAM-1, CD44, VLA-5, LFA-1, syndecan-1,…) and indirect interactions (soluble factors) between MM plasma cells and BM-MSCs result in constitutive abnormalities in BM-MSCs. In particular, MM BM-MSCs express less CD106 and fibronectin and more DKK1, IL-1β and TNF-α as compared with normal BM-MSCs. In order to gain a global view of the differences between BM-MSCs from MM patients and healthy donors, we used gene expression profiling to identify genes associated to the transformation of MM BM-MSCs.
|
| |
|
| Overall design |
BM-MSCs were isolated from 3 healthy donors and 4 untreated multiple myeloma patients. Total RNA from BM-MSCs was exctracted and hybridyzed on Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array. Amplification, hybridization and scanning were done according to standard Affymetrix protocols (www.affymetrix.com). CEL files were normalized with RMA method.
|
| |
|
| Contributor(s) |
André T, Meuleman N, Stamatopoulos B, De Bruyn C, Pieters K, Bron D, Lagneaux L |
| Citation(s) |
23555770 |
| |
| Submission date |
Mar 13, 2012 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Thibaud André |
| E-mail(s) |
thibaud.andre@bordet.be
|
| Organization name |
ULB - Jules Bordet Insitute
|
| Lab |
Laboratory of Clinical Cell Therapy
|
| Street address |
Boulevard de Waterloo, 121
|
| City |
Brussels |
| ZIP/Postal code |
1000 |
| Country |
Belgium |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (7)
|
|
| Relations |
| BioProject |
PRJNA153503 |
Less...
More...